Method for identifying self-compatibility of diploid potato

A diploid and potato technology, applied in the field of genetic breeding, can solve the problems of time-consuming and labor-intensive identification methods, inaccurate representation, and affecting plants

Active Publication Date: 2020-03-20
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fruit setting of the plant is affected by various internal and external factors, such as low pollen activity due to immature pollen development, high or low temperature during pollination will affect the fruit setting of the plant, and potato, as a flowering plant, may be caused by wind blowing or tweezers not cleaned during pollination. The result of cross-fertilization between adjac...

Method used

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  • Method for identifying self-compatibility of diploid potato
  • Method for identifying self-compatibility of diploid potato
  • Method for identifying self-compatibility of diploid potato

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0102] A method for identifying whether diploid potatoes are self-compatible, comprising the following steps:

[0103] (1) Genome resequencing of parent materials RH89-039-16 and PI 225689:

[0104] RH89-039-16 is a self-compatible diploid potato material, and PI 225689 is a self-incompatible diploid potato material. After crossing these two materials as parents, the offspring appeared self-compatibility Separated from the self-incompatibility trait, in order to accurately identify the self-compatibility phenotype of the offspring, the genome resequencing of the parent material RH89-039-16 and PI 225689 was performed to obtain the sequence information of the two materials.

[0105] (2) Develop molecular markers co-segregated with StSCI gene in parent material RH89-039-16 and PI 225689:

[0106] Comparing the sequence information of the chromosomal segment containing the StSCI gene in the parent material RH89-039-16 and PI 225689, it was found that a 538bp (SEQ ID NO.2) nucleo...

Embodiment 2

[0129] A method for screening molecular markers for identifying whether diploid potatoes are self-compatible, comprising the following steps:

[0130] (1) Obtain the genome sequence information of the parent material:

[0131] The self-compatible diploid potato RH89-039-16 and the self-incompatible diploid potato PI225689 were used as parental materials to obtain hybrid offspring, and the genomes of RH89-039-16 and PI225689 were resequenced to obtain genome sequence information;

[0132] (2) Screening for single-base differential sites in parental materials:

[0133] By analyzing the nucleotide sequence of the StSCI gene and the linked nucleotide sequence of the StSCI gene, it was found that RH89-039-16 at 58030614 bp on chromosome 12 is a T base, while the corresponding position on the PI 225689 material is a C base;

[0134] The StSCI gene has the nucleotide sequence shown in SEQ ID NO.1; the genetic distance of gene linkage is less than 20cM;

[0135] (3) Design KASP mo...

Embodiment 3

[0140] A method for screening molecular markers for identifying whether diploid potatoes are self-compatible, comprising the following steps:

[0141] (1) Obtain the genome sequence information of the parent material:

[0142] The self-compatible diploid potato RH89-039-16 and the self-incompatible diploid potato PI225689 were used as parental materials to obtain hybrid offspring, and the genomes of RH89-039-16 and PI225689 were resequenced to obtain genome sequence information;

[0143] (2) Screening for indel markers in parental materials:

[0144] By analyzing the nucleotide sequence of the StSCI gene and the linked nucleotide sequence of the StSCI gene, it was screened that at chromosome 12 58136285, RH89-039-16 lacked 11 bp of base sequence relative to PI 225689;

[0145] The StSCI gene has the nucleotide sequence shown in SEQ ID NO.1; the genetic distance of gene linkage is less than 20cM;

[0146] (3) Design InDel molecular markers:

[0147] Appropriate primer fragm...

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Abstract

The invention provides a method for identifying whether a diploid potato is self-compatible, and relates to the technical field of genetic breeding. The method is to identify whether StSCI gene in thediploid potato is transcribed and expressed. The invention also discloses a method for adopting molecular markers to identify whether the StSCI gene is expressed and for screening molecular markers.The method for screening molecular markers comprises: obtaining genomic sequence information of a parent material, screening differential sites of the parent material, screening molecular markers, andidentifying whether the screened molecular markers are available. Identification of self-compatibility of diploid potatoes by adopting the screened molecular markers has the advantages that the identification workload is small, a lot of time is saved, and the identification results are not affected by the environment, are accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to a method for identifying whether diploid potatoes are self-compatible, a method for screening molecular markers for identifying whether diploid potatoes are self-compatible, reagents, kits and identification method. Background technique [0002] During diploid potato breeding, diploid potatoes are often self-incompatible, and when pollen from itself or the same S haplotype as the plant falls on the stigma, the pollen tube cannot extend all the way to ovule completion The fertilization process, therefore pollen identical to its own S haplotype is incapable of producing seeds in diploid potato plants. In order to achieve seed breeding in diploid potato breeding, some studies have focused on modifying the self-incompatibility defect of diploid potato through gene editing. [0003] But the identification of self-compatible diploid potato materials has been difficult. At p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/29C12Q1/6858C12Q1/6809
CPCC12Q1/6895C07K14/415C12Q1/6858C12Q1/6809C12Q2600/13C12Q2600/158C12Q2600/156C12Q2531/113C12Q2565/125A01H1/045A01H1/022A01H5/04A01H6/827
Inventor 马玲尚轶黄三文张春枝高冬丽
Owner YUNNAN NORMAL UNIV
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