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A track regeneration type DNA walker and its application

A walker and track technology, applied in the direction of recombinant DNA technology, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of limiting the number of walker steps and unfavorable walker operation, so as to increase the number of walking steps, enhance Continuity of operation and improvement of work efficiency

Active Publication Date: 2021-05-28
SHANDONG UNIV
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  • Description
  • Claims
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Problems solved by technology

However, in the existing design of DNA walkers, as the walking chain walks, the track for the walking chain to walk will be gradually exhausted due to the replacement of the chain or the consumption of hydrolysis, which severely limits the walking ability of the walker. The number of steps taken, which is not conducive to the continuous operation of the walker along the track

Method used

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  • A track regeneration type DNA walker and its application
  • A track regeneration type DNA walker and its application
  • A track regeneration type DNA walker and its application

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Embodiment 1

[0051] 1.1 Chemicals and reagents

[0052] All oligonucleotides were purchased from Shanghai Sangon Biotechnology Co., Ltd., and their sequences are listed in Table 1. Exonuclease III (Exo III) used at a concentration of 100000 U mL -1 ) was purchased from New England Biolabs Ltd. (Beijing, China). All chemicals used were of analytical purity and were used without further purification. Throughout the experiment, 18.25 MΩcm Milli-Q ultra-pure water was used.

[0053] Table 1. Oligonucleotide sequences used in this work

[0054]

[0055]

[0056] [a] GenBank: AF086833.2. [b] GenBank: KU182911.1. [c] GenBank: FJ968794.1. [d] GenBank: MF540570.1. [e] GenBank: MH121167.1.

[0057] 1.2 Instruments

[0058] UV-Vis absorption spectra were recorded with a U-2910 UV-Vis spectrophotometer (Japan). Fluorescence emission spectra were collected on a Hitachi F-7000 fluorescence spectrophotometer (Japan). Its excitation wavelength is 488nm, and its emission spectrum ranges from 5...

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Abstract

The disclosure belongs to the technical field of DNA walker construction, and in particular relates to a track-regenerating DNA walker and its application. This disclosure proposes a concept of a track-regenerating DNA walker and verifies it. The walker uses a detachable track consisting of roadbed and rails. Its road-based chains are functionalized on gold nanoparticles, while a large number of rail chains are free in solution and will hybridize with road-based chains to construct tracks. As the walker walks along the track, the rail chains are digested and the roadbed chains are released, and the released roadbed chains continue to combine with the rail chains in the solution to form new tracks. The constant replenishment of the track allows the machine to keep walking. This machine has great advantages in signal enrichment, so it can be used for sensitive detection of Ebola virus gene fragments in serum.

Description

technical field [0001] The disclosure belongs to the technical field of DNA walker construction, and in particular relates to a DNA walker with reproducible orbit, the walker and its application in biosensing. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present disclosure, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] DNA molecular machines are a class of molecular machines assembled from DNA that perform machine-like behaviors at the micro or nano scale. Benefiting from the unique properties of DNA molecules, such as strict complementary base pairing, excellent recognition ability, and high programmability, DNA molecular machines can be precisely controlled and finely manipulated in response to various stimuli. To date, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/682
CPCC12Q1/682C12Q2525/301C12Q2563/137C12Q2563/107C12Q2565/519
Inventor 姜玮逄寒徐晓文
Owner SHANDONG UNIV
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