Construction method of mouse ventricular aneurysm model

Abstract

The invention discloses a construction method of a mouse ventricular aneurysm model, comprising the following steps: anesthetizing a mouse by inhaling isoflurane without mechanical ventilation; forming a small skin incision in the left chest to expose the intercostal space of the most violent cardiac beating after the mouse enters a relaxed state and does not have respiratory depression and dysphoria; forming a small hole between the ribs by using tissue forceps and hemostatic forceps, opening the pleura and pericardium, and slightly extruding the thorax of the mouse; ligating at a position 4-4.5 mm away from the origin of the coronary artery by adopting an absorbable 6-0 suture; ligating the left anterior descending branch and the half myocardium together; immediately putting the heart into the thoracic cavity after ligation, manually exhausting, and suturing and knotting. According to the method, a simple, repeatable and stable mouse ventricular aneurysm model can be manufactured, the model forming rate is high, the method can be used for researching before ventricular aneurysm formation and researching the internal mechanism of mouse ventricular aneurysm formation under different strains and in different states, and a theoretical basis is provided for treatment and prevention of ventricular aneurysm.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing a mouse ventricular aneurysm model. Background technique [0002] Cardiovascular disease can lead to high mortality and morbidity. At present, acute myocardial infarction (MI) is still the number one "killer" of human beings. Left ventricular aneurysm (LVA) is one of the complications of myocardial infarction due to the movement disorder and abnormal movement of part of the left ventricular wall area due to myocardial infarction, which can reduce the left ventricular ejection fraction (LVEF) and fractional shortening (LVFS) , thus causing left heart failure, severe cases can also cause some life-threatening adverse events, such as massive formation of thrombus, severe arrhythmia and cardiac rupture. [0003] At present, according to the size of ventricular aneurysm and its impact on cardiac function, different treatment methods are selected clinically. Sm...

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Problems solved by technology

[0004] In the past, the animal models of ventricular aneurysm can only be applied to rats, rabbits, sheep, etc., and because the modeling rate is not high, the therapeutic effect of drugs and polymer materials can only be studied after the appearance of ventricular aneurysm, such as " Chinese Journal of Thoracic and Cardiovascular Surgery, Volume 19, Issue 3, June 2012, "Establishment of Left Ventricular Reconstruction Model of Rat Ventricular Aneurysm" and the doctoral dissertation of Xinjiang Medical University, "Myocardial Aneurysm Formation Process in Rabbits after Acute Myocardial Infarction" According to the technical content recorded in "Experimental Research on Mechanics and MMP-9 Changes", the above schemes cannot achieve early intervention in the process of model making, and the screening process is a necessary process, and a certain proportion of samples are eliminated in the screening process

At present, there is no stable model making method that can be applied to mice, and because the mouse strain is much larger than other model animals, there is an urgent need for a stable mouse ventricular aneurysm model making method

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