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Correction method of nitrous oxide isotope [delta]15N

A calibration method, a technique for nitrous oxide, used in measuring devices, material analysis by electromagnetic means, instruments, etc.

Active Publication Date: 2020-04-10
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

At present, there is no establishment of N 2 O gas isotope value δ 15 Accurate measurement and correction method of N

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  • Correction method of nitrous oxide isotope [delta]15N
  • Correction method of nitrous oxide isotope [delta]15N

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Embodiment 1

[0037] Example 1 Nitrous oxide gas isotope δ 15 N correction method

[0038] (1) Pseudomonas aureus caused by denitrifying bacteria ( Pseudomonas aureofaciens ) culture and culture medium detection

[0039] against Pseudomonas aureus ( Pseudomonas aureofaciens ) culture, the formula of the medium is: 30 g / L Tryptone Soy Broth Agar (TSB), 10mM KNO 3 , 7.5 mM (NH 4 ) 2 SO 4 , 36mM KH 2 PO 4 , 440ml medium is placed in a 500ml container. In addition, the rotation speed of the shaker was improved to 160 rpm, which was found to be more suitable for the growth of the bacteria, and the culture period was 5-10 days. After 5 days of continuous culture, take 1ml of bacterial liquid and add sulfonamide for color reaction, if the bacterial liquid does not change color, it means NO 3 - The conversion is complete, and the subsequent stage of the test can be carried out. If the color changes, seal the culture bottle and continue to culture on a shaker, and carry out color reacti...

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Abstract

The invention provides a correction method of nitrous oxide gas isotope [delta]15N. According to the invention, four standard nitrates or nitrites, namely, USGS32, USGS34, IAEA-NO-3 and RSIL-N7373 ofpseudomonas aureofaciens are utilized for gradually reducing nitrate (NO3<->) into nitrite (NO2<->), nitric oxide (NO) and nitrous oxide (N2O) through a denitrification method to obtain four referenceN2O gases with standard [delta]15Nbulk and [delta]15N[alpha] values, linear regression is carried out on an actual measurement value and a true value to obtain a correction equation, and [delta]15N[alpha] and [delta]15Nbulk of an N2O sample to be detected are obtained on the basis of the correction equation, so that the SP value of the N2O gas is calculated. The source of N2O in the N2O synthesisroute can be known through the SP value, and technical support is provided for emission reduction of greenhouse gas N2O in the atmosphere.

Description

technical field [0001] The invention relates to the technical field of gas isotope measurement and correction, in particular to a nitrous oxide gas isotope δ 15 N correction method. Background technique [0002] Nitrous oxide (N 2 O) is an important greenhouse gas with both a greenhouse effect and a major source of nitric oxide (NO) involved in the catalytic cycle of ozone destruction. Although its atmospheric abundance is about carbon dioxide (CO 2 ), but its radiative efficiency is about that of CO 2 300 times, and stay in the atmosphere for a long time. In the last century, N 2 The atmospheric concentration of O increased steadily at a rate of 0.2–0.3% per year, and this increase was mainly due to the increase in soil available nitrogen after agricultural expansion and intensification of production. Analysis of N by Stable Isotope Technique 2 O isotopomers are essential for understanding N 2 The O source mechanism is of great help, thus providing the N 2 O emissi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 丁军军李玉中毛丽丽李巧珍徐春英
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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