Thermostable streptomyce, microbial soil reducing agent, and preparation method and application of microbial soil reducing agent

A technology of microorganism and reducing agent, applied in the field of microbial soil reducing agent and its preparation, high temperature resistant Streptomyces, can solve the problems of soil pH imbalance, reducing crop quality, long time, etc., to promote crop growth, improve crop quality, improve The effect of soil pH

Active Publication Date: 2020-04-14
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes a long time for the traditional high-temperature stuffy shed, and it usually takes about a month to completely

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045] The present invention provides the preparation method of the microbial soil reducing agent described in the above-mentioned technical scheme, is characterized in that, comprises the following steps: (1) Streptomyces vulgaris thermo, Streptomyces grisea and Bacillus subtilis are respectively inoculated on the activation medium Cultivate; (2) the activated Streptomyces vulgaris, Streptomyces cinerea and Bacillus subtilis are respectively inoculated in respective seed culture medium, and cultivated on a shaking table to obtain Streptomyces vulgaris thermos, Streptomyces cinerea and Streptomyces cinerea respectively Seed liquid and Streptomyces grisea seed liquid; (3) The seed liquids of Streptomyces vulgaris, Streptomyces grisea and Bacillus subtilis obtained in step (2) are respectively inoculated in a liquid fermentation medium, and fermented to obtain a fermented (4) mixing the fermented liquids of Streptomyces vulgaris, Streptomyces grisea and Bacillus subtilis obtained...

Embodiment 1

[0064] (1) Strain activation: inoculate Streptomyces vulgaris Sanju-11 and Streptomyces grisea Sanju-09 in Gao's No. 1 medium, and Bacillus subtilis Sanju-07 in beef extract peptone medium to activate.

[0065] (2) Seed liquid preparation: inoculate activated Streptomyces vulgaris, Streptomyces grisea, and Bacillus subtilis with their respective seed liquid culture medium in proportions of 10%, 10%, and 10% by volume respectively in the respective activated In the culture medium, cultivate under a shaker at 40°C and 150rpm, culture Streptomyces vulgaris and Streptomyces grisea for 48 hours, and Bacillus subtilis for 36 hours to obtain Streptomyces vulgaris thermale, Streptomyces grisea seed liquid and Streptomyces grisea respectively. Streptomyces rubrum seed solution.

[0066] (3) Expanded culture by liquid fermentation: inoculate the seed fermentation liquid with an inoculum size of 8% by volume in the liquid fermentation medium.

[0067] Liquid fermentation medium inocula...

Embodiment 2

[0071] Experimental design: 9 sheds were set up in the experiment, each of which was regarded as 1 treatment, divided into 3 treatments in total, arranged in random blocks, and protected rows were set around.

[0072] Process A blank control;

[0073] Treatment B conventional irrigation high temperature stuffy shed;

[0074] Process C high-temperature stuffy shed+the microbial soil reducing agent prepared by the preparation method of embodiment 1;

[0075] Fertilization management: On July 20, 2017, the stuffy shed treatment was carried out, and treatment A was not treated; treatment B was routinely irrigated and stuffed shed; treatment C was 2L / 666.7m 2The amount of application of the microbial soil reducing agent obtained in Example 1 was diluted 400 times and then sprayed. Cucumbers were planted after 15 days in the stuffy shed. When the cucumbers matured, the incidence and growth conditions were detected. The straw decomposing effect during the stuffy shed period was de...

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Abstract

The invention belongs to the technical field of soil reducing agent preparation, particularly relates to a microbial soil reducing agent, and a preparation method and an application thereof, and provides a microbial soil reducing agent capable of improving soil acidity and alkalinity imbalance, and a preparation method and an application thereof. The microbial soil reducing agent comprises, by volume, Streptomyces thermophilus, Streptomyces griseorubens and Bacillus subtilis; and the effective viable count ratio of the Streptomyces thermophilus to the Streptomyces griseorubens to the bBacillussubtilis is (5-7):(7-9):(4-6). The microbial soil reducing agent thermostable bacterium can adjust soil nutrient balance and improve soil acidity and alkalinity, and also can inhibit soil-borne diseases, promote crop growth and improve crop quality.

Description

technical field [0001] The invention belongs to the technical field of soil reducing agents, and in particular relates to a high temperature-resistant streptomyces, microbial soil reducing agent and its preparation method and application. Background technique [0002] In recent years, with the continuous growth of people's demand for vegetables, facility agriculture has also developed rapidly and has become the most important way of vegetable supply. However, in the process of the development of facility agriculture, the original ecological environment of the soil has gradually changed. With the increase of the multiple cropping index, problems such as serious soil-borne diseases, imbalance of soil nutrients, and reduced crop quality have appeared. These problems have greatly restricted the safety of vegetables. develop. [0003] Research at home and abroad shows that the current solution to the soil problems of facility agriculture mainly depends on chemical control, but c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/465C09K101/00C09K109/00
CPCC09K17/14C09K2101/00C09K2109/00C12N1/20C12N1/205C12R2001/465
Inventor 郭小红朱春苗翟修彩李锡明李肖宇刘玉珍黄娟钱潘攀
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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