A kind of drug-loaded synovial fluid additive and its preparation method and application

A joint synovial fluid and loaded technology, which is applied in the direction of drug combination, pharmaceutical formula, antipyretic, etc., can solve the problems that the synthesis preparation technology has not yet been established, and achieve the benefits of large-scale production, lower surface energy, and prolong lubrication time Effect

Active Publication Date: 2022-08-09
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no public report that fluorinated graphene is used to enhance hyaluronic acid, improve its mechanical properties, and use it to load anti-inflammatory drugs and treat joint inflammation, and the relevant synthesis preparation technology has not yet been established.

Method used

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  • A kind of drug-loaded synovial fluid additive and its preparation method and application
  • A kind of drug-loaded synovial fluid additive and its preparation method and application
  • A kind of drug-loaded synovial fluid additive and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of Tris-HCl buffered saline solution (TBS solution) with pH=8.5: Accurately weigh 0.12114 g of Tris-HCl solid, add 20 mL of ultrapure water to dissolve, and adjust the pH to 8.5 with concentrated HCl.

[0040] (2) Accurately weigh 1 g of HA, dissolve 50 mg of FGO in 100 mL of TBS solution with pH=8.5, and sonicate for 40 min; add 500 mg of DA-HCl, and use HCl to adjust the pH to 8.5; the oxygen content of FGO is 45%, and the fluorine content is 55%.

[0041] (3) After mixing evenly, stir, protect from light, and heat at 60 °C for 24 h under nitrogen protection. After the reaction, the above solution was put into a dialysis bag and dialyzed in deionized water for 1 day. It was transferred into a beaker, sealed with filter paper, and freeze-dried to obtain a dopamine, hyaluronic acid-modified fluorinated graphene sample (FGO-PDA-HA).

Embodiment 2

[0043] (1) Prepare Tris-HCl buffered saline solution (TBS solution) with pH=8.0: Accurately weigh the Tris-HCl solid, add ultrapure water to dissolve, and adjust the pH to 8.0 with concentrated HCl.

[0044](2) Accurately weigh 2 g of HA, dissolve 100 mg of FGO in 200 mL of TBS solution with pH=8.0, and sonicate for 40 min; add 500 mg of DA, and use HCl to adjust the pH to 8.0; the oxygen content of FGO is 95%, The fluorine content is 5%.

[0045] (3) After mixing evenly, stir, protect from light, and heat at 80 °C for 7 h under nitrogen protection. After the reaction, the above solution was put into a dialysis bag and dialyzed in deionized water for 1 day. It was transferred into a beaker, sealed with filter paper, and freeze-dried to obtain a dopamine, hyaluronic acid-modified fluorinated graphene sample (FGO-PDA-HA).

Embodiment 3

[0047] (1) Prepare Tris-HCl buffered saline solution (TBS solution) with pH=10: Accurately weigh the Tris-HCl solid, add ultrapure water to dissolve, and adjust the pH to 10 with concentrated HCl.

[0048] (2) Accurately weigh 1 g of HA, dissolve 100 mg of FGO in 200 mL of TBS solution with pH=10, and sonicate for 40 min; add 10 mg of DA-HCl, and use HCl to adjust the pH to 9.5; the oxygen content of FGO is 5%, and the fluorine content is 95%.

[0049] (3) After mixing evenly, stir, protect from light, and heat at 25 °C for 36 h under helium protection. After the reaction, the above solution was put into a dialysis bag and dialyzed in deionized water for 1 day. It was transferred into a beaker, sealed with filter paper, and freeze-dried to obtain a dopamine, hyaluronic acid-modified fluorinated graphene sample (FGO-PDA-HA).

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Abstract

The invention discloses a drug-loaded synovial fluid additive. Using fluorinated graphene, dopamine and hyaluronic acid / sodium hyaluronate as main raw materials, a one-step method is used to synthesize polydopamine coupling, hyaluronic acid / hyaluronic acid The sodium-modified fluorinated graphene nano-drug carrier is used as a composite additive to improve the lubricating properties of joint synovial fluid after adsorbing drugs for the treatment of arthritis. The preparation method of the technology is simple, the compound additive has good biocompatibility, can significantly reduce the degradation rate of hyaluronic acid, the loaded drugs can effectively treat joint inflammation, and greatly improve the friction reduction and antiwear properties of joint synovial fluid performance, thereby extending the life of the synovial fluid.

Description

technical field [0001] The invention belongs to the technical fields of mechanical engineering and biological engineering, and particularly relates to a drug-loaded synovial fluid additive and a preparation method and application thereof. Background technique [0002] Arthritis is a common chronic disease. The wear and inflammation of articular cartilage caused by the failure of synovial fluid lubrication is an important cause of arthritis (see Adv. Funct. Mater., 2018, 1807559; Adv. Health. Mater., 2018, 1701463). When arthritis occurs, the content and molecular weight of hyaluronic acid in synovial fluid will decrease, and patients need to inject HA to achieve cartilage repair and viscosity compensation. However, the injected hyaluronic acid has insufficient compressive strength, poor abrasion resistance, and rapid degradation in vivo at low frequency and high load. It requires frequent injections to achieve good results, and repeated frequent injections increase the pain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/52A61K47/04A61K31/728A61K31/196A61P19/02A61P29/00
CPCA61K47/52A61K47/02A61K31/728A61K31/196A61P19/02A61P29/00A61K2300/00
Inventor 公培伟刘哲彭景一赵凯翟明珠李慧许文宇
Owner QUFU NORMAL UNIV
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