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Enzymatic gelatin-based hydrogel with controllable blood-coagulation and antibacterial properties and preparation method of hydrogel

A hydrogel and gelatin-based technology, applied in the field of biomaterial preparation, can solve problems such as thrombosis and wound healing, and achieve the effects of prolonging the service life, avoiding the risk of drug resistance, and having good biocompatibility

Active Publication Date: 2020-04-28
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, if the biomaterial is designed with strong blood clotting properties, it may lead to the occurrence of blood clots in some applications
And if the coagulation performance is too weak, there will be a hidden danger that the wound will not heal in some applications
Therefore, it is still a great challenge to design multifunctional biomaterials that can be adjusted to meet the different needs of the human body.

Method used

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  • Enzymatic gelatin-based hydrogel with controllable blood-coagulation and antibacterial properties and preparation method of hydrogel
  • Enzymatic gelatin-based hydrogel with controllable blood-coagulation and antibacterial properties and preparation method of hydrogel
  • Enzymatic gelatin-based hydrogel with controllable blood-coagulation and antibacterial properties and preparation method of hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Weigh 5 g of gelatin into a centrifuge tube, add 1.5 ml of phosphate buffer solution, place in a constant temperature water bath, adjust the temperature to 50°C, and stir until completely dissolved to obtain a gelatin solution;

[0033] 2) Weigh 0.04 g of cucurbituril [7] and 0.02 g of polylysine into a centrifuge tube, add 2 ml of phosphate buffer to dissolve, and place the mixed solution in ultrasonic for 10 min to make it evenly dispersed;

[0034] 3) Weigh 0.04 g of transglutaminase into a centrifuge tube, add 1 ml of phosphate buffer to dissolve, and obtain a transglutaminase solution;

[0035] 4) Mix the gelatin solution obtained in step 1) with the mixed solution obtained in step 2), and stir to make them evenly mixed;

[0036] 5) Add the enzyme solution obtained in step 3) to the solution in step 4) that was mixed uniformly above, and stir to make it evenly mixed, and transfer the above solution to a hydrogel mold, place it in a constant temperature oscillato...

Embodiment 2

[0038] 1) Weigh 5 g of gelatin into a centrifuge tube, add 1.5 ml of phosphate buffer solution, place in a constant temperature water bath, adjust the temperature to 50°C, and stir until completely dissolved to obtain a gelatin solution;

[0039] 2) Weigh 0.02 g of polylysine into a centrifuge tube, add 2 ml of phosphate buffer to dissolve, and obtain a polylysine solution;

[0040] 3) Weigh 0.04 g of transglutaminase into a centrifuge tube, add 1 ml of phosphate buffer to dissolve, and obtain a transglutaminase solution;

[0041] 4) Mix the gelatin solution obtained in step 1) with the polylysine solution obtained in step 2), and stir to make them evenly mixed;

[0042] 5) Add the enzyme solution obtained in step 3) to the solution in step 4) that was mixed uniformly above, and stir to make it evenly mixed, and transfer the above solution to a hydrogel mold, place it in a constant temperature oscillator, and adjust the temperature to At 45°C, react for 8 h to obtain the hydr...

Embodiment 3

[0044] 1) Weigh 5 g of gelatin into a centrifuge tube, add 1.5 ml of phosphate buffer solution, place in a constant temperature water bath, adjust the temperature to 50°C, and stir until completely dissolved to obtain a gelatin solution;

[0045] 2) Weigh 0.06 g of cucurbituril [7] and 0.02 g of polylysine into a centrifuge tube, add 2 ml of phosphate buffer to dissolve, and place the mixed solution in ultrasonic for 10 min to make it evenly dispersed;

[0046] 3) Weigh 0.04 g of transglutaminase into a centrifuge tube, add 1 ml of phosphate buffer to dissolve, and obtain a transglutaminase solution;

[0047] 4) Mix the gelatin solution obtained in step 1) with the mixed solution obtained in step 2), and stir to make them evenly mixed;

[0048] 5) Add the enzyme solution obtained in step 3) to the solution in step 4) that was mixed uniformly above, and stir to make it evenly mixed, and transfer the above solution to a hydrogel mold, place it in a constant temperature oscillato...

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Abstract

The invention relates to a method for preparing a biomaterial with controllable antibacterial and blood-coagulation properties, good biocompatibility and certain self-repairing properties by using by-product gelatin extracted from leather waste as a raw material. The method uses glutamine transaminase to modify the gelatin, the modified gelatin, polylysine and cucurbit[7]uril are mixed to preparethe biomaterial that meets the needs of human bodies in different environments. The method has a simple preparation process and is easy to operate, no harmful chemical substances are added in the whole process, no secondary pollution is caused to the environment, and the biomaterial is expected to have better application prospects in the future.

Description

technical field [0001] The invention relates to the modification of gelatin and the application field of synthesizing multifunctional hydrogels, in particular to a preparation method of biological materials meeting different needs of human bodies. Background technique [0002] A qualified biomaterial often has certain mechanical properties, self-healing properties, antibacterial properties, and biocompatibility, etc. Designing and synthesizing such multifunctional biomaterials is still a research hotspot. So far, many multifunctional biomaterials have been reported. However, due to the complex environment of the human body, biomaterials with different needs for different parts of the human body have different requirements for antibacterial properties and blood control. If the antibacterial property is too strong, it may have certain side effects on the human body, and if the antibacterial property is too weak, it will easily cause bacterial infection. And bacterial infectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C08J3/075C08J3/24C08L89/00C08L77/04C08K5/21
CPCA61L26/0038A61L26/0047A61L26/0019A61L26/008A61L26/0066C08J3/075C08J3/24C08J2389/00C08J2477/04C08K5/21A61L2300/404A61L2300/418C08L89/00C08L77/04
Inventor 王学川甘婷朱兴王园园王雪莹李霄
Owner SHAANXI UNIV OF SCI & TECH
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