Bacterial strain with plant soil-borne disease resisting effect and application thereof

A technology for soil-borne diseases and plants, applied in the direction of bacteria, plant growth regulators, plant growth regulators, etc., can solve problems such as environmental pollution and unsatisfactory control measures, and achieve reduction of agricultural pollution, easy expansion of industrial production, and good The effect of developing application prospects

Active Publication Date: 2020-04-28
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to: aim at the severe occurrence of root rot, rust rot, fusarium wilt, blight, bacterial wilt, etc. in the current soil-borne diseases, and the control measures such as pesticides, crop rotation, and soil chemical treatment have unsatisfa

Method used

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  • Bacterial strain with plant soil-borne disease resisting effect and application thereof
  • Bacterial strain with plant soil-borne disease resisting effect and application thereof
  • Bacterial strain with plant soil-borne disease resisting effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 The acquisition of Bacillus velezensis CGMCC No.15320:

[0027] The three-year-old Panax notoginseng rhizosphere soil was collected from Yunnan Province, and 5g of the soil sample was weighed after removing the stones, put into a triangular bottle filled with 45mL sterile water, and shaken for 20min, that is, 10 -1 soil suspension; serially diluted to 10 -2 、10 -3 、10 -4 、10 -5Afterwards, each dilution was spread on a separation plate, inverted, and incubated at 26°C for 1-5 days. Observation of culture, after the growth of a single colony, it was transferred to LB medium for culture, and the strain CGMCC No.15320 was obtained.

Embodiment 2

[0028] Example 2 Identification of Bacillus velezensis CGMCC No.15320

[0029] 1 Observation of Morphological Characteristics

[0030] The strain Bacillus velezensis CGMCC No.15320 cultured for 1-5 days was opened to take pictures against a black background. The colony is off-white, with folds on the surface, no pigmentation, and the outermost periphery is off-white ( figure 1 ).

[0031] 2 16S rRNA sequence analysis

[0032] After the genomic DNA of the strain was extracted by liquid nitrogen grinding, primers: 16s F: 5'-AGAGTTTGATCCTGGCTCAG-3'; 16s R: 5'-AAGGAGGTGATCCAGCCGCA-3' were used for PCR amplification. After gel electrophoresis, it was sent to Bioengineering (Shanghai) Co., Ltd. for sequencing. The full length of the sequence is 1524bp (see SEQ). The resulting sequence was submitted to the GenBank database for BLAST analysis and comparison, and it was found that the strain with higher homology to CGMCC No.15320 belonged to Bacillus velezensis, and the strain sequ...

Embodiment 3

[0033] The promoting action of embodiment 3 Bacillus velezensis (Bacillus velezensis) bacterial suspension to Radix Notoginseng, cucumber, capsicum, corn seed germination

[0034] Inoculate the above-mentioned bacterial strains on LB medium plates, culture them upside down at 26-30°C for 1-5 days, activate the bacterial strains, use distilled water to prepare the activated bacterial strains into a bacterial suspension, and use a UV spectrophotometer to dilute at a wavelength of 600nm The concentration of the bacterial suspension is: 0.1-0.8, diluted 100 times, and set aside. Distilled water was used as a control, and each group was repeated three times, with 100 seeds in each repetition, and each group was soaked in 50 mL of bacterial suspension dilution for 1-5 hours. Seeds were filtered through a sieve, cultured at 20-25°C for 20 days, and the germination rate of Panax notoginseng seeds was counted; 24 hours at 20-25°C, and the germination rate of cucumber seeds was counted;...

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Abstract

The invention relates to bacillus velezensis which has beneficial effect on plants and can be used for preventing and treating soil-borne diseases of plants and a microbial inoculum of bacillus velezensis, and belongs to the technical field of biological pesticides. The strain is separated from three-year-old panax notoginseng root soil, is identified as bacillus velezensis, and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 15320. The bacillus velezensis, a bacterial strain suspension or a bacterial strain fermentation broth or a bacterial strain fermentation broth extracting solution or a microbial inoculum containing the bacterial strain not only can promote seed germination and growth of plants to a certain extent, butalso can remarkably reduce the occurrence rate of soil-borne diseases of the plants, particularly the occurrence rate of the soil-borne diseases of the plants on a continuous cropping field. The microbial inoculum containing the strain can also be mixed with an organic fertilizer to be prepared into a bacterial fertilizer for use, and a wide application prospect is brought to the field of biological prevention and control of plant diseases.

Description

Technical field: [0001] The present invention relates to a kind of biocontrol bacterium Bacillius velezensis and its application, in particular to a strain of Bacillius velezensis which has a probiotic effect on plant growth and its role in the prevention and treatment of plant soil-borne diseases, especially The application in continuous cropping plant soil-borne diseases belongs to the technical field of biological pesticides. Background technique: [0002] In the past 20 years, my country has gradually realized the transformation from traditional agriculture to modern agriculture. According to the data published in the China Agricultural Yearbook in 2015, the planting area of ​​protected areas in my country has exceeded 3.8 million hm 2 , 20.3526 million hm of high value-added vegetables 2 . The planting area of ​​important Chinese medicinal materials such as ginseng and Panax notoginseng ranks first in the world. In 2015, the planting area of ​​ginseng reached 2,100 ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12R2001/07C12N1/205Y02A40/10
Inventor 章朦玥张怡轩
Owner SHENYANG PHARMA UNIVERSITY
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