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Rapid method and system for directly reconstructing structured light illumination super-resolution image in airspace

A technology of structured light illumination and super-resolution images, which is applied in the direction of material analysis, optics, and material analysis through optical means, and can solve problems such as complex operations and difficulties in real-time dynamic observation of super-resolution microscopy

Active Publication Date: 2020-04-28
MGI TECH CO LTD
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Problems solved by technology

[0003] Aiming at the difficulty of real-time dynamic observation of super-resolution microscopy caused by the current structured light illumination super-resolution image reconstruction method due to complex operation and serious time-consuming, the present invention proposes a method for directly reconstructing structured light illumination super-resolution images in the airspace The fast method (SDR method for short), its operation steps are greatly simplified compared with the traditional method, the steps are reduced from the traditional 5 steps to 2 steps, and it is a basic multiplication and addition operation, which does not require Fourier transform operations, so the reconstruction speed Greatly improve

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  • Rapid method and system for directly reconstructing structured light illumination super-resolution image in airspace
  • Rapid method and system for directly reconstructing structured light illumination super-resolution image in airspace
  • Rapid method and system for directly reconstructing structured light illumination super-resolution image in airspace

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Embodiment 1

[0104] This embodiment is based on the super-resolution image reconstruction of the projected SIM of digital micromirror DMD modulation and LED lighting, and is realized by the following steps:

[0105] Step 1. Use image 3 The shown projection SIM super-resolution microscope system based on digital micromirror DMD modulation and LED lighting, firstly, the 405nm wavelength LED beam enters the beam splitter 2 and illuminates the digital micromirror DMD, and the structured light modulated by the digital micromirror DMD transmits Excessive beam prism 2 exits, collimates through collimator lens 4 and enters microscopic objective lens 8, and microscopic objective lens 8 shrinks the structured light stripes and projects them on its focal plane;

[0106]Step 2, placing the bovine pulmonary artery endothelial cell sample on the stage 9 and adjusting it to the focal plane of the microscope objective lens, the structured light generated by the miniature projection illuminates the sample...

Embodiment 2

[0122] This embodiment is based on the SLM modulation of the spatial light modulator and the super-resolution image reconstruction of the interferometric SIM of the zero-order vortex half-wave plate polarization control:

[0123] Step 1. Use Figure 4 The shown interferometric SIM super-resolution microscopy system based on spatial light modulator SLM modulation and zero-order vortex half-wave plate polarization control, with a 532nm wavelength laser incident polarizing beam splitter 4 and vertically irradiating the spatial light modulator SLM6, Produces multi-level diffracted beams with vertical linear polarization. The converged zero-order and high-order beams of multi-order diffraction are blocked by a spatial filter 8, and only ±1-order diffracted beams are retained. Subsequently, the ±1st-order diffracted light whose polarization state is changed by the zero-order vortex half-wave plate 9 enters the microscope objective lens 13, and the two beams of diffracted light inte...

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Abstract

The invention relates to a rapid method and system for directly reconstructing a structured light illumination super-resolution image in an airspace, wherein the rapid method and system can be widelyapplied to researches in the fields of biology, medicine, material science, microelectronics and the like. Therefore, a problem that the super-resolution microscopic real-time dynamic observation is difficult due to complex operation and serious time consumption of a current structured light illumination super-resolution image reconstruction method is solved. Compared with a traditional frequency-domain SIM super-resolution image reconstruction method, the provided method has the advantages that the operation is completely carried out in the space domain and thus the processes of frequency spectrum separation, frequency spectrum movement, frequency spectrum fusion and the like are avoided and time-consuming multi-times forward / inverse Fourier transform operations are avoided, so that a problem of artifact noise caused by frequency spectrum operation is completely solved; a demodulation matrix for super-resolution reconstruction can be generated in advance, the related operations are only multiplication and addition operations and thus the process is simple and easy to implement, so that the reconstruction speed of the SIM super-resolution image is greatly increased.

Description

technical field [0001] The invention relates to a fast method for directly reconstructing super-resolution images illuminated by structured light in airspace, which can be widely used in research in the fields of biology, medicine, material science, microelectronics and the like. Background technique [0002] The spatial resolution of traditional optical microscopes is limited by the diffraction limit of light, which can only reach the order of half the wavelength of light, which greatly limits the application range of optical microscopy, such as imaging of structures in living biological cells. Realizing high spatial resolution imaging has always been one of the important research topics in the field of optical microscopy. By labeling the sample with the help of fluorescent molecules, the signal-to-noise ratio and contrast of imaging in optical microscopy can be significantly improved. Based on the intensity response mechanism of fluorescent molecules to illumination light...

Claims

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Application Information

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IPC IPC(8): G01N21/64G02B21/00
CPCG01N21/6458G02B21/0052G02B21/0076
Inventor 姚保利但旦雷铭周兴千佳赵天宇
Owner MGI TECH CO LTD
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