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Incubation liquid capable of promoting growth of in vitro tumor tissue capillaries, and preparation method of incubation liquid capable of promoting growth of in vitro tumor tissue capillaries

A tumor tissue and microvessel technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of providing sufficient nutrients for tumor growth, tumor tissue necrosis, ablation, etc., to promote tumor tissue microangiogenesis, promote The effect of spawning or growing

Pending Publication Date: 2020-05-01
南京普恩瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After transplantation into immunodeficient mice, the limited blood vessels cannot provide enough nutrients for the continued growth of the tumor, which can easily lead to necrosis and ablation of tumor tissue in mice

Method used

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  • Incubation liquid capable of promoting growth of in vitro tumor tissue capillaries, and preparation method of incubation liquid capable of promoting growth of in vitro tumor tissue capillaries
  • Incubation liquid capable of promoting growth of in vitro tumor tissue capillaries, and preparation method of incubation liquid capable of promoting growth of in vitro tumor tissue capillaries
  • Incubation liquid capable of promoting growth of in vitro tumor tissue capillaries, and preparation method of incubation liquid capable of promoting growth of in vitro tumor tissue capillaries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation method of incubation solution for promoting growth of microvessels in isolated tumor tissue

[0032] The steps of the preparation method include: (1) adding 50 μl of fetal bovine serum (FBS) to the EP tube, then adding growth factors of 5 μl of Ang-2, 20 μl of VEGF-164, and 25 μl of EGF respectively, mixing well, and preparing A growth factor suspension with a final concentration of 4.75 μg / ml, in which the concentrations of Ang-2, VEGF-164, and EGF are 50 μg / ml, 5 μg / ml, and 5 μg / ml respectively; All experimental instruments and consumables need to be pre-cooled to 4°C in advance, including pipette tips, pipette guns, and 96-well plates; add 50 μl of Matrigel to each culture well of the 96-well plate, and then add the prepared 50 μl Finally, add penicillin and streptomycin to the growth factor suspension, and mix well to make the final concentration of penicillin and streptomycin 100U / ml, and the incubation solution for promoting the growth of mi...

Embodiment 2

[0033] Example 2: Preparation method of incubation solution for promoting growth of microvessels in isolated tumor tissue

[0034] The steps of the preparation method include: (1) adding 50 μl of fetal bovine serum (FBS) to the EP tube, then adding growth factors of 5 μl of Ang-2, 20 μl of VEGF-164, and 25 μl of EGF respectively, mixing well, and preparing A growth factor suspension with a final concentration of 1.9 μg / ml was formed, in which the concentrations of Ang-2, VEGF-164, and EGF were 20 μg / ml, 2 μg / ml, and 2 μg / ml respectively; All experimental instruments and consumables need to be pre-cooled to 4°C in advance, including pipette tips, pipette guns, and 96-well plates; add 50 μl of Matrigel to each culture well of the 96-well plate, and then add the prepared 100 μl Finally, add penicillin and streptomycin to the growth factor suspension, and mix well to make the final concentration of penicillin and streptomycin 100U / ml, and the incubation solution for promoting the ...

Embodiment 3

[0035] Example 3: Preparation method of incubation solution for promoting growth of microvessels in isolated tumor tissue

[0036] The steps of the preparation method include: (1) adding 50 μl of fetal bovine serum (FBS) to the EP tube, then adding growth factors of 5 μl of Ang-2, 20 μl of VEGF-164, and 25 μl of EGF respectively, mixing well, and preparing A growth factor suspension with a final concentration of 14.50 μg / ml, in which the concentrations of Ang-2, VEGF-164, and EGF are 100 μg / ml, 10 μg / ml, and 10 μg / ml respectively; All experimental instruments and consumables need to be pre-cooled to 4°C in advance, including pipette tips, pipette guns, and 96-well plates; add 50 μl of Matrigel to each culture well of the 96-well plate, and then add the prepared 10 μl Finally, add penicillin and streptomycin to the growth factor suspension, and mix well to make the final concentration of penicillin and streptomycin 100U / ml, and the incubation solution for promoting the growth o...

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Abstract

The invention discloses incubation liquid capable of promoting growth of in vitro tumor tissue capillaries, and a preparation method of the incubation liquid capable of promoting growth of in vitro tumor tissue capillaries. The incubation liquid comprises matrigel, fetal calf serum, growth factors and antibiotics, wherein the volume ratio of the matrigel to the fetal calf serum to the growth factors is 1 to (0.2-1.0) to (0.2-1.0); and the final concentration of the antibiotics in the incubation liquid is 100-400U / ml. The incubation liquid capable of promoting growth of in vitro tumor tissue capillaries increases the modeling success rate of tumor tissue, shortens the modeling time, can perform in vivo drug effect screening in the shortest time, can service the market better, can enable clinicians to obtain an optimized screening scheme faster, and provides treatment service for patients.

Description

technical field [0001] The invention relates to the technical field of angiogenesis promotion, in particular to an incubation solution for promoting microvascular growth of isolated tumor tissue and a preparation method thereof. Background technique [0002] Human-derived tumor xenograft model (Patient-Derived tumor Xenograft, PDX) is a human-derived xenograft model established by directly transplanting patient's tumor tissue into immunodeficient mice. Most primary tumors have good predictability of clinical efficacy. [0003] The clinically derived tumor tissue tumor mass in PDX ensures that the basic shape of the tumor cells remains unchanged, and the stromal cells, fibroblasts, microvascular network, and three-dimensional space in the tumor mass are consistent with the tumor tissue in the body. However, at present, the overall success rate of PDX model modeling for different cancer types is low, about 25%, and the construction time is long and the success rate is unstabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2533/90C12N2501/17C12N2501/165C12N2501/11
Inventor 朱燕萍王志强郝艳鹏王涛只朱宁颖
Owner 南京普恩瑞生物科技有限公司
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