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Helicobacter pylori detection kit and preparation method of the kit

A technology of Helicobacter pylori and detection kit, which is applied in the biological field, can solve the problems of inaccurate results, influence of detection, and difficulty in distinguishing observation, etc., and achieves the effects of improving sensitivity and improving enzyme activity.

Pending Publication Date: 2020-05-01
欧迪玛(湖北)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex composition of saliva, some components or other types of microorganisms will have a certain impact on the detection, and because the number of Helicobacter pylori is small, the urease reaction is often not obvious, making the color change of the indicator difficult to distinguish and observe, resulting in inaccurate results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a Helicobacter pylori detection kit, which includes a reaction solution and an indicator test paper, the reaction solution includes citric acid-sodium citrate buffer solution, nickel chloride and urea at pH 3.0, the indicator The test paper is methyl red test paper.

[0031] Its preparation method is as follows:

[0032] S1. Weigh 21.01g of citric acid and dissolve it in deionized water, and set the volume to 1L to obtain a 0.1mol / L citric acid solution, weigh 29.41g of sodium citrate solution and dissolve it in deionized water, and set the volume to 1L to obtain 0.1mol / L sodium citrate solution, above-mentioned citric acid solution and sodium citrate solution are mixed by volume ratio 93:7, obtain the citric acid-sodium citrate buffer solution of pH3.0;

[0033] S2, take by weighing 1.30g of anhydrous nickel chloride and dissolve it in deionized water, place in a 100mL volumetric flask, and set the volume to 100mL to obtain a nickel ch...

Embodiment 2

[0040] The present embodiment provides a Helicobacter pylori detection kit, which includes a reaction solution and an indicator test paper, the reaction solution includes pH4.0 citric acid-sodium citrate buffer, nickel chloride and urea, the indicator The test paper is bromocresol purple test paper.

[0041] Its preparation method is as follows:

[0042] S1. Weigh 21.01g of citric acid and dissolve it in deionized water, and set the volume to 1L to obtain a 0.1mol / L citric acid solution, weigh 29.41g of sodium citrate solution and dissolve it in deionized water, and set the volume to 1L to obtain 0.1mol / L sodium citrate solution, above-mentioned citric acid solution and sodium citrate solution are mixed by volume ratio 131:69, obtain the citric acid-sodium citrate buffer solution of pH4.0;

[0043] S2, take by weighing 1.56g of anhydrous nickel chloride and dissolve it in deionized water, place in a 100mL volumetric flask, and set the volume to 100mL to obtain a nickel chlor...

Embodiment 3

[0050] The present embodiment provides a Helicobacter pylori detection kit, which includes a reaction solution and an indicator test paper, the reaction solution includes citric acid-sodium citrate buffer solution, nickel chloride and urea at pH 5.0, the indicator The test paper is litmus paper.

[0051] Its preparation method is as follows:

[0052]S1. Weigh 21.01g of citric acid and dissolve it in deionized water, and set the volume to 1L to obtain a 0.1mol / L citric acid solution; weigh 29.41g of sodium citrate solution and dissolve it in deionized water, and set the volume to 1L to obtain 0.1mol / L sodium citrate solution, above-mentioned citric acid solution and sodium citrate solution are mixed by volume ratio 41:59, obtain the citric acid-sodium citrate buffer solution of pH5.0;

[0053] S2, take by weighing 2.59g of anhydrous nickel chloride and dissolve it in deionized water, place in a 100mL volumetric flask, and set the volume to 100mL to obtain a nickel chloride so...

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Abstract

The invention discloses a helicobacter pylori detection kit and a preparation method of the kit. The kit comprises a reaction solution and an indicator paper. The reaction solution includes the citricacid at a pH of 3.0-5.0, sodium citrate buffer, nickel chloride and urea. The indicator test paper can detect the change of pH in the range of pH 4.5 to 7.0. The kit of the invention can be used fordetecting the infection of helicobacter pylori in saliva. The reagent has the advantages of simple preparation, low cost, no harm, pain, toxic side effects and taboos to the human body. The method issimple and fast, and the kit has high sensitivity, thereby being suitable for popularization and application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Helicobacter pylori detection kit and a preparation method thereof. Background technique [0002] Helicobacter Pylori (HP) is an important cause of chronic active gastritis, peptic ulcer, gastric cancer and mucosa-associated lymphoma. Studies have shown that the Helicobacter pylori isolated from the dental plaque of patients with gastritis, after culture and application of DNA restriction endonuclease, it is clear that these Helicobacter pylori are the same as those in the stomach, and it is confirmed that there are also Helicobacter pylori parasitism in the oral cavity. The second storage place behind the stomach. [0003] Since Marshall and Warren successfully isolated Helicobacter pylori in 1983. Scholars at home and abroad have conducted extensive research on this. The current methods for detecting Hp can be classified into two categories. [0004] Invasive method: it is ne...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/04G01N2333/195
Inventor 郭东辉
Owner 欧迪玛(湖北)生物科技有限公司
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