Application of spermine in preparing drug for treating periprosthetic osteolysis

A technology of periprosthetic bone and spermine, which can be used in drug combinations, pharmaceutical formulations, bone diseases, etc., can solve problems that have not been fully elucidated, and achieve the effects of reducing bone loss, reducing osteolysis, and inhibiting osteolysis.

Pending Publication Date: 2020-05-05
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the clinical benefits of spermine on bone metabolism have been reported in several studies, the mode of action of its anti-osteoclastic activity and its relation to inflammatory osteolysis after joint replacement has not been fully elucidated, the present invention is thus

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  • Application of spermine in preparing drug for treating periprosthetic osteolysis
  • Application of spermine in preparing drug for treating periprosthetic osteolysis
  • Application of spermine in preparing drug for treating periprosthetic osteolysis

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with specific examples, but the examples are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0036] 1. Materials and methods

[0037] 1. Materials

[0038] 1.1 Reagents and experimental equipment

[0039] 1.1.1 Main Drugs and Reagents

[0040]Spermin (Spermin), purchased from Sigma, USA; TRAP staining kit, ethylenediaminetetraacetic acid (EDTA), purchased from Sigma, USA; paraformaldehyde, PBS, DAB chromogen, hematoxylin, eosin, anhydrous Ethanol, distilled water, 10% chloral hydrate. RT-PCR kit was purchased from Invitrogen, USA; titan...

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Abstract

The invention discloses application of spermine in preparing a drug for treating periprosthetic osteolysis. Periprosthetic osteolysis occurs after artificial joint replacement. Meanwhile, the invention discloses a pharmaceutical preparation for treating periprosthetic osteolysis. For the application and the pharmaceutical preparation disclosed by the invention, animal experiments prove that the spermine can reduce expression of RANKL, p65, TNF-alpha and IL-1 beta, relieves osteolysis and bone loss induced by titanium particles, confirmed that the spermine has the significant inhibition effecton the osteolysis induced by abrasion particles and can be used as a new means of drug intervention of periprosthetic osteolysis after artificial prosthesis replacement, and thus a new way for treatment of periprosthetic osteolysis is provided.

Description

technical field [0001] The invention belongs to the technical field of new uses of medicines, and in particular relates to the application of spermine in the preparation of medicines for treating periprosthetic osteolysis. Background technique [0002] At present, artificial joint replacement (AJR) is considered to be the most effective surgical method for the treatment of various end-stage bone and joint diseases. However, with the increase in the number of artificial joint replacement cases and the prolongation of time, the number of patients requiring revision due to joint replacement failure also increased. After 15 to 20 years of prosthesis replacement, 10 to 15% of prostheses will fail. Studies have shown that aseptic loosening caused by peri-prosthetic osteolysis (PPO) is the main reason for shortening the use time of joint prosthesis. PPO is a complex lesion with multiple factors, and its exact mechanism is still not fully understood, but the biological response ca...

Claims

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Application Information

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IPC IPC(8): A61K31/132A61P19/08
CPCA61K31/132A61P19/08
Inventor 耿德春郁彬清柏家祥葛高然徐耀增杨惠林
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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