Bioluminescent probe for detecting sulfite as well as preparation method and application of bioluminescent probe

Abstract

The invention provides a bioluminescent probe for detecting sulfite as well as a preparation method and application of the bioluminescent probe. The probe is a compound shown as a formula I (See the specification for reference), or salt thereof, or a stereoisomer thereof, wherein n is an integer from 1 to 5, and R1, R2, R3, R4, R5 and R6 are respectively and independently selected from hydrogen, C1-C6 alkyl, C1-C6 alkoxy, halogen, hydroxyl, carboxyl, amino and nitro. The bioluminescent probe provided by the invention has high selectivity and high responsiveness to sulfite ions in sulfite, cansingly and accurately select sulfite, has very high sensitivity during detection, can detect sulfite with very low concentration, and is used for precision detection of sulfite. Meanwhile, the bioluminescent probe can also be used for carrying out dynamic visual analysis on sulfite accumulation caused by exogenous sulfite and sulfite oxidase deficiency at a living body level, is beneficial to clinical detection of sulfite, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a bioluminescent probe for detecting sulfite, a preparation method and application thereof. Background technique [0002] Sulfite is a kind of food additive that has been widely used in the world for a long time. It can be used as food bleaching agent and preservative; it can inhibit non-enzymatic browning and enzymatic browning, prevent food browning, and keep fruits from black It can also prevent fresh shrimp from forming black spots; it is also a very effective antibacterial agent in acidic medium. Since sulfite plays an important role in food storage, it is widely used in the food field. Therefore, sulfite can easily enter the human body along with food. Although there is a sulfite oxidase in the human body, it can catalyze the combination of sulfite and oxygen to generate non-toxic SO 4 2- ; However, if the content of sulfite in the human body is...

AI Technical Summary

Problems solved by technology

At present, the probes used to detect sulfite are constantly developing, but there are many problems, such as: the detection limit does not meet the requirements, the detection sensitivity is low, the reaction time is long, the anti-interference ability is poor, and it cannot be used for dynamic visualization of living bodies detection etc.

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