Droplet-type digital PCR kit for COVID-19 nucleic acid test and application of kit

A coronavirus and micro-droplet technology, applied in the direction of resistance to vector-borne diseases, microbial measurement/inspection, biochemical equipment and methods, etc., can solve droplet loss, slow PCR reaction speed, and low-load virus detection sensitivity Insufficient and other problems, to achieve the effect of high stability
CN111118225APending Publication Date: 2020-05-08SUZHOU PRECIGENOME LTD CO
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
SUZHOU PRECIGENOME LTD CO
Publication Date
2020-05-08

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Abstract

The invention provides a droplet-type digital PCR kit for COVID-19 nucleic acid test and an application of the kit. A reaction solution in the kit contains primers and a probe for detecting a COVID-19ORF1ab gene and a COVID-19 N gene, and primers and a probe for detecting human RNA reference genes, and two ends of each probe are labeled with fluorescent groups. The kit fully utilizes the characteristic of a small sample volume of microfluidic treatment, and realizes rapid heating and cooling of a sample, the maximum heating and cooling rate of the system can reach 10 DEG C / s, at the same time, the detection sensitivity to COVID-19 is 1 to 2 orders of magnitude higher than that of a traditional qPCR, and under the condition of a low virus concentration, the detection repeatability is better than that of the traditional qPCR.
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Description

technical field

[0001] The invention belongs to the technical field of virus detection, and in particular relates to a droplet digital PCR kit for nucleic acid detection of novel coronavirus and its application. Background technique

[0002] Coronavirus is a type of RNA virus with an envelope and a linear single-stranded positive strand genome, with a diameter of about 80-120nm. Currently, it only infects humans, mice, pigs, cats, dogs, and poultry vertebrates. The novel coronavirus (COVID-19) has been confirmed as a new variant, which can cause viral pneumonia, with fever and dry cough in mild cases, dyspnea and even shock in severe cases. The method widely used in the market to detect the current novel coronavirus (COVID-19) is the method of nucleic acid extraction and purification followed by quantitative PCR. Its detection sensitivity is limited, and it is easily inhibited by different chemical substances from the patient's body in the sample, thereby affecting the dete...

Claims

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