Method for extracting ovotransferrin from pigeon eggs

A technology of ovotransferrin and pigeon eggs, which is applied in the direction of preparation methods of transferrin and peptides, chemical instruments and methods, etc., can solve the problems of low extraction rate, limitation of large-scale industrial production and extraction of ovotransferrin, and low purity problems, to achieve high extraction rates

Inactive Publication Date: 2020-05-15
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing extraction methods, there are problems of low extraction rate and low purity; thus limiting the large-scale

Method used

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  • Method for extracting ovotransferrin from pigeon eggs
  • Method for extracting ovotransferrin from pigeon eggs
  • Method for extracting ovotransferrin from pigeon eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 extracts the method for ovotransferrin from pigeon eggs

[0036] (1) Separate 200 g of egg white liquid from pigeon eggs, add 400 g of water to the egg white liquid for dilution, stir magnetically for 60 min, then centrifuge at 6000 r / min for 20 min at 4°C, filter with gauze, and take the supernatant;

[0037] (2) Then add FeCl to the supernatant 3 ·6H 2 O 3.24g, sodium bicarbonate 2.52g, sodium chloride 5.256g, then magnetically stirred for 120min;

[0038] (3) Add dehydrated ethanol to the supernatant again to make the ethanol volume concentration in the supernatant reach 43%, stir and homogeneously leave standstill for 30 min, and centrifuge to obtain precipitation 1 and filtrate;

[0039] (4) Continue adding absolute ethanol to the filtrate, so that the volume concentration of ethanol in the filtrate reaches 59%, stir evenly, let stand overnight, and centrifuge to obtain precipitate 2.

[0040] (5) Combine Precipitation 1 and Precipitation 2, wash wi...

Embodiment 2

[0041] Embodiment 2 extracts the method for ovotransferrin from pigeon eggs

[0042] (1) Separate 200 g of egg white liquid from pigeon eggs, add 300 g of water to the egg white liquid for dilution, stir magnetically for 70 min, then centrifuge at 5500 r / min for 25 min at 4°C, filter with gauze, and get the supernatant;

[0043] (2) Then add FeCl to the supernatant 3 ·6H 2 O 3.24g, sodium bicarbonate 2.52g, sodium chloride 5.256g, then magnetically stirred for 100min;

[0044] (3) adding dehydrated ethanol to the supernatant to make the volume concentration of ethanol in the supernatant reach 40%, stirring and homogenizing, leaving standstill for 30 min, and centrifuging to obtain the precipitate 1 and the filtrate;

[0045] (4) Continue adding absolute ethanol to the filtrate, so that the volume concentration of ethanol in the filtrate reaches 65%, stir evenly, let stand overnight, and centrifuge to obtain precipitate 2.

[0046] (5) Combine Precipitation 1 and Precipitati...

Embodiment 3

[0047] Embodiment 3 extracts the method for ovotransferrin from pigeon eggs

[0048] (1) Separate 200 g of egg white liquid from pigeon eggs, add 600 g of water to the egg white liquid to dilute, stir magnetically for 50 min, then centrifuge at 6500 r / min for 15 min at 4°C, filter with gauze, and take the supernatant;

[0049] (2) Then add FeCl to the supernatant 3 ·6H 2 O 3.24g, sodium bicarbonate 2.52g, sodium chloride 5.256g, then magnetically stirred for 150min;

[0050] (3) adding dehydrated ethanol to the supernatant to make the ethanol volume concentration in the supernatant reach 45%, stirring and homogenizing, leaving standstill for 30 min, and centrifuging to obtain the precipitate 1 and the filtrate;

[0051] (4) Continue adding absolute ethanol to the filtrate, so that the volume concentration of ethanol in the filtrate reaches 55%, stir evenly, let stand overnight, and centrifuge to obtain precipitate 2.

[0052] (5) Combine Precipitation 1 and Precipitation 2, w...

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Abstract

The invention discloses a method for extracting ovotransferrin from pigeon eggs. The method comprises the following steps: (1) separating an egg white solution from pigeon eggs, diluting the egg whitesolution, performing sufficient stirring, and performing centrifugal filtration to obtain supernatant; (2) adding FeCl3.6H2O, sodium bicarbonate and sodium chloride into the supernatant, and continuing to perform sufficient stirring; (3) adding ethyl alcohol into the supernatant to enable the volume concentration of ethyl alcohol in the solution to be 40-45 percent, and performing uniform stirring, standing and centrifuging to obtain precipitate 1 and filtrate; (4) keeping on adding ethyl alcohol into the filtrate to enable the volume concentration of the ethyl alcohol in the filtrate to be 55-65 percent, and performing uniform stirring, standing and centrifuging to obtain precipitate 2; and (5) mixing the precipitate 1 and the precipitate 2, washing the mixture with the ethyl alcohol, re-dissolving the mixture by adding water, mixing and stirring the mixture with resin, and performing suction filtration, dialysis and freeze-drying to obtain the ovotransferrin. The brand-new method for extracting the ovotransferrin from pigeon eggs has relatively high extraction rate and purity.

Description

technical field [0001] The invention relates to the technical field of ovotransferrin preparation, in particular to a method for extracting ovotransferrin from pigeon eggs. Background technique [0002] Ovotransferrin, also known as conalbumin, is an important single-chain glycoprotein in poultry egg white, accounting for about 12% of the total protein content of egg white. Its protein molecule is composed of 686 amino acid residues, and its molecular weight ranges from 78 to 80 kDa. It can combine with metal ions such as iron, copper or zinc to cause a large change in the conformation of ovotransferrin. Ovotransferrin has a variety of physiological functions, such as enhancing the body's immunity, promoting the growth of lymphocytes, and preventing or inhibiting the invasion of pathogenic bacteria. Ovotransferrin can also transport iron ions to the parts of the body that need iron, thereby promoting the body's absorption of iron and nutrients, and effectively preventing an...

Claims

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Application Information

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IPC IPC(8): C07K14/79C07K1/14C07K1/34
CPCC07K14/79
Inventor 杨娟冯晓燕曾晓房白卫东赵文红董浩刘巧瑜于立梅
Owner ZHONGKAI UNIV OF AGRI & ENG
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