Surface-enhanced Raman-based quick detection method for flavin protein progeny
A surface-enhanced Raman and flavoprotein technology, applied in the field of rapid detection of flavoprotein prosthetic groups, can solve the problems of high quality and performance requirements of operating instruments, high testing costs, complicated operations, etc., and achieve high sensitivity and convenient detection. , the effect of simple operation process
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[0019] Embodiment 1: This embodiment provides that the present invention provides a method for rapid detection of flavoprotein prosthetic groups based on surface-enhanced Raman, comprising the following steps:
[0020] Step 1, dissolving the prosthetic group of the flavoprotein to be tested in water, preparing aqueous solutions with different concentration gradients and mixing them uniformly;
[0021] Step 2, taking 2 μL of the solution obtained in Step 2 and dripping it onto the silver nanorod array substrate to concentrate the solution to obtain a detection solution;
[0022] In step 3, the detection solution obtained in step 3 is subjected to SERS detection, the laser power is set to 30 mW, the integration time is 10 s, and the SERS spectrum of the detection solution is measured; figure 1 It is the spectrogram of different concentrations of flavoprotein prosthetic groups based on surface-enhanced Raman. It can be seen that the present invention has good sensitivity and can ...
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