Surface-enhanced Raman-based quick detection method for flavin protein progeny

A surface-enhanced Raman and flavoprotein technology, applied in the field of rapid detection of flavoprotein prosthetic groups, can solve the problems of high quality and performance requirements of operating instruments, high testing costs, complicated operations, etc., and achieve high sensitivity and convenient detection. , the effect of simple operation process

Pending Publication Date: 2020-05-19
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

The above method is not only complicated to operate, but also has high requirements for the quality of the personnel operating the instrument and the performance of the instrument itself, and the test cost is relatively high

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  • Surface-enhanced Raman-based quick detection method for flavin protein progeny
  • Surface-enhanced Raman-based quick detection method for flavin protein progeny

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Embodiment 1

[0019] Embodiment 1: This embodiment provides that the present invention provides a method for rapid detection of flavoprotein prosthetic groups based on surface-enhanced Raman, comprising the following steps:

[0020] Step 1, dissolving the prosthetic group of the flavoprotein to be tested in water, preparing aqueous solutions with different concentration gradients and mixing them uniformly;

[0021] Step 2, taking 2 μL of the solution obtained in Step 2 and dripping it onto the silver nanorod array substrate to concentrate the solution to obtain a detection solution;

[0022] In step 3, the detection solution obtained in step 3 is subjected to SERS detection, the laser power is set to 30 mW, the integration time is 10 s, and the SERS spectrum of the detection solution is measured; figure 1 It is the spectrogram of different concentrations of flavoprotein prosthetic groups based on surface-enhanced Raman. It can be seen that the present invention has good sensitivity and can ...

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Abstract

The invention discloses a surface enhanced Raman spectroscopy (SERS)-based method for quickly detecting a flavin protein prothetic group. The method comprises the following steps: directly collectingan SERS signal of a flavin protein auxiliary group; the method provided by the invention is simple to operate and label-free, and the detection capability on the flavin protein progeny can reach 0.1 mg/L, so that the detection sensitivity is higher and more stable, the influence of external temperature and a sample on qualitative detection is greatly reduced, the detection is more convenient, andthe operation process is simple. And a new method can be provided for real-time monitoring of biological medicine and food production.

Description

technical field [0001] The invention relates to a method for rapidly detecting prosthetic groups of flavin protein based on surface-enhanced Raman, in particular to a method for detecting flavin mononucleotide (FMN). Background technique [0002] Flavoproteins are binding proteins with flavin nucleotides as prosthetic groups, which participate in many biological processes, including photosynthesis, bioluminescence, apoptosis, DNA repair, and elimination of reactive oxygen species. Flavoproteins usually contain flavin coenzymes such as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). They participate in the transfer of electrons from substrates to electron acceptors in the bound state with enzyme proteins. In organisms, it is synthesized through riboflavin and ATP through the action of riboflavin kinase. Dysregulation of flavin coenzymes has been linked to many diseases such as Parkinson's disease, migraine and multiple sclerosis. In addition, FMN can als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 田康振聂新明田亚平
Owner XUZHOU NORMAL UNIVERSITY
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