Injectable bionic repair material for temporomandibular disease and preparation method and application of injectable bionic repair material
A technology for temporomandibular joint and repair materials, which is applied in the field of injectable bionic repair materials for temporomandibular joint disc defects and its preparation, can solve the problem of limiting the application and promotion prospects of filling repair materials for temporomandibular joint disc defects, and can not meet the needs of filling. Problems such as regular defects and lack of stent shape can achieve the effects of regeneration and repair, excellent mechanical properties, and excellent mechanical properties
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Embodiment 1
[0061] This embodiment provides an injectable biomimetic repair material for temporomandibular joint disc defects. The biomimetic repair material includes A component and B component, wherein the A component includes I-type gelatin, Hyaluronic acid (MAHA), methacrylic anhydride-modified chondroitin sulfate (MACS) and photoinitiator; the B component includes four-arm polyglycolic acid succinimidyl ester (PEG-NHS).
[0062] The mass ratio of the type I gelatin, the methacrylic anhydride-modified chondroitin sulfate (MACS) and the methacrylic anhydride-modified hyaluronic acid (MAHA) is 5-20:1-4:4. The mass ratio of the type I gelatin to the four-arm polyglycolic acid succinimidyl ester (PEG-NHS) is 3:1. The type I gelatin is derived from fish scales. The photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone. The A component and the B component in the biomimetic repair material are mixed for use.
[0063] This embodiment also provides the application of a bion...
Embodiment 2
[0066] refer to Figure 12 , the present embodiment provides a method for preparing an injectable biomimetic repair material for temporomandibular joint disc defects, the method comprising the following steps:
[0067] S1. Type I gelatin (viscosity range 2.88mPa·s~5.28mPa·s), methacrylic anhydride-modified hyaluronic acid (MAHA) and methacrylic anhydride-modified chondroitin sulfate (MACS) according to mass ratio 5~20:1~4:4 dissolved in deionized water, and adding photoinitiator (2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone), adjust the pH and fully dissolve , to obtain a composite aqueous solution, namely the A component;
[0068] S2. Dissolving four-arm polyglycolic acid succinimidyl ester (PEG-NHS) with a relative molecular weight of 20 kDa in deionized water for later use to obtain component B.
[0069] The mass ratio of the type I gelatin to the four-arm polyglycolic acid succinimidyl ester (PEG-NHS) is 3:1. The A component and the B component in the biomimetic...
Embodiment 3
[0079] This embodiment provides an injectable biomimetic repair material for temporomandibular joint disc defects. The biomimetic repair material includes A component and B component, wherein the A component includes I-type gelatin, Hyaluronic acid (MAHA), methacrylic anhydride-modified chondroitin sulfate (MACS) and photoinitiator; the B component includes four-arm polyglycolic acid succinimidyl ester (PEG-NHS). The photoinitiator is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone.
[0080] In this example, the preparation method of the injectable bionic repair material for temporomandibular joint disc defect is as follows: type I gelatin (viscosity 2.88mPa·s) derived from fish scales, MACS, and MAHA were prepared according to the ratio of 15:1:4. Mass ratio Dissolve in deionized water, add photoinitiator I2959, adjust pH, dissolve in water bath to obtain component A; dissolve PEG-NHS in deionized water to obtain component B.
[0081] In this example, the application of...
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