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Application of human tpt1/tctp ​​gene in preparation of antineoplastic drugs

A gene and drug technology, applied in the field of preparation of anti-tumor drugs, can solve the problems that there are no related reports

Active Publication Date: 2021-04-13
福州载基生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the association between TPT1 / TCTP gene and other thyroid cancers and thymoma

Method used

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  • Application of human tpt1/tctp ​​gene in preparation of antineoplastic drugs
  • Application of human tpt1/tctp ​​gene in preparation of antineoplastic drugs
  • Application of human tpt1/tctp ​​gene in preparation of antineoplastic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Construction of TPT1 lentiviral vector and establishment of stably transfected cell lines

[0017] 1. siRNA target design

[0018] The nucleotide sequence of human TPT1 (NM_001286272.1) was queried at the National Center for Biological Information (NCBI), and 12 pairs of effective siRNA targets were designed for the coding sequence region using DSIR software.

[0019]

[0020] 2. Plasmid construction of pLVX-shRNA2-Puro-TPT1 and negative control pLVX-shRNA2-Puro-NC

[0021] According to the above siRNA sequence (taking SEQ ID NO: 1, 2, 3 as an example), design corresponding complementary single-stranded DNA, and add BamHI and EcoRI restriction sites at both ends.

[0022]

[0023] Annealing: Anneal the upstream and downstream fragments of single-stranded shRNA to form double-stranded DNA fragments, which are then ligated to the digested vector.

[0024] Annealing reaction system:

[0025]

[0026] Amplification program: 95°C, 5 min; cool at room te...

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Abstract

The application of the human TPT1 / TCTP gene in the preparation of antitumor drugs belongs to the field of biotechnology. The invention constructs human TPT1 / TCTP gene small molecule interference RNA, human TPT1 / TCTP gene interference nucleic acid construct, human TPT1 / TCTP gene interference lentivirus and discloses their applications. The siRNA provided by the present invention or the nucleic acid construct containing the siRNA sequence, and the lentivirus can specifically inhibit the expression of human TPT1 / TCTP gene, especially the lentivirus, which can efficiently infect target cells and efficiently inhibit TPT1 / TCTP gene expression in target cells. The expression of TCTP gene can inhibit the growth of tumor cells and promote the apoptosis of tumor cells, which is of great significance in the treatment of tumors.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of human TPT1 / TCTP gene in the preparation of antitumor drugs. Background technique [0002] Tumor translation control protein (TPT1 or TCTP) is a growth-related protein that can promote growth and resist apoptosis. Blocking the expression of this protein can inhibit the malignant phenotype of tumor cell lines. There is no report about the association between TPT1 / TCTP gene and other thyroid cancers and thymoma. In the prior art, similar genes are used to construct human gene small molecule interference RNA, human gene interference nucleic acid construct, human gene interference lentivirus and disclose their applications. Provide siRNA or a nucleic acid construct containing the siRNA sequence, lentivirus can specifically inhibit the expression of human genes, especially lentivirus, can efficiently infect target cells, efficiently inhibit gene expression i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K48/00A61P35/00
CPCA61K31/713A61P35/00
Inventor 王巧文邱德辉谢翔峰
Owner 福州载基生物科技有限公司