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Method for improving enzyme activity of glutathion peroxidase in lactic acid bacteria

A technology of glutathione peroxide and lactic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, can solve the problem of low enzyme activity and achieve enhanced enzyme activity and expression The effect of volume increase

Pending Publication Date: 2020-06-05
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is simple and easy to implement, and the effect is remarkable, which solves the problem that the enzyme activity of the existing glutathione peroxidase is generally low

Method used

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  • Method for improving enzyme activity of glutathion peroxidase in lactic acid bacteria
  • Method for improving enzyme activity of glutathion peroxidase in lactic acid bacteria
  • Method for improving enzyme activity of glutathion peroxidase in lactic acid bacteria

Examples

Experimental program
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Embodiment 1

[0046] Step 1, Activation of Strains

[0047] Inject 0.3 mL of the MRS broth medium sterilized by high temperature and high pressure into the freeze-dried tube of Lactobacillus rhamnosus, and gently blow it with a pipette gun to fully dissolve it into a bacterial suspension. Aspirate the bacterial suspension, put them all into 2 test tubes, and cultivate them under the specified conditions of the strains. After the activation is successful, in order to improve the activity of the strains, inoculate each strain into the MRS broth medium, and place them in a constant temperature incubator. Cultivate at 37°C for 24 hours, and activate three generations continuously.

[0048] Step 2, selenium-enriched culture of Lactobacillus rhamnosus

[0049] Prepare 0.17mg / mL sodium selenite solution and MRS liquid medium and pre-sterilize it. When in use, add sodium selenite solution into MRS liquid medium and dilute to a sodium selenite concentration of 20 μg / mL. Inoculate 1 mL of the above...

Embodiment 2-7

[0052] In embodiment 2-7, Lactobacillus rhamnosus is replaced by Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus plantarum respectively, other conditions are identical with embodiment 1 .

Embodiment 8-14

[0054] The concentration of sodium selenite was adjusted to 40 μg / mL, 60 μg / mL, 80 μg / mL, 100 μg / mL, 120 μg / mL, 140 μg / mL, 160 μg / mL, and other conditions were the same as in Example 1.

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Abstract

The invention provides a method for improving the enzyme activity of glutathion peroxidase in lactic acid bacteria. The method comprises the following steps: Step 1, activated culture is performed onthe lactic acid bacteria to obtain lactic acid bacteria solution; Step 2, the lactic acid bacteria solution is inoculated into a liquid medium containing sodium selenite, and undergoes salt stress orheat stress selenium-rich culture. According to the method, the sodium selenite is utilized to carry out selenium-rich culture on the lactic acid bacteria, and after lactic acid bacteria undergo the salt stress or heat stress selenium-rich culture, the enzyme activity of the glutathion peroxidase in the lactic acid bacteria is improved.

Description

technical field [0001] The invention relates to glutathione peroxidase, in particular to a method for improving the enzyme activity of lactic acid bacteria glutathione peroxidase. Background technique [0002] Glutathione peroxidase (GSH-Px) is an important selenium-containing peroxide decomposing enzyme in organisms. The active center of GSH-Px is selenocysteine. Its main function is to cooperate with vitamins to protect cells from peroxide damage and remove harmful free radicals in the body. It is an enzyme that resists peroxidation in cells. An important component of the protective system. GSH-Px can catalyze reduced glutathione (GSH) into oxidized glutathione (GSSG), reduce toxic peroxides to non-toxic hydroxyl compounds, and promote H 2 o 2 Decomposition, thereby protecting the structure and function of the cell membrane from the interference and damage of peroxides. GSH-Px mainly includes four types: cytoplasmic GSH-Px, plasma GSH-Px, phospholipid hydroperoxide GSH...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12R1/225C12R1/245C12R1/46C12R1/25C12R1/23
CPCC12N1/20C12N1/38
Inventor 徐颖贺佳璇许牡丹张婷缑敬轩
Owner SHAANXI UNIV OF SCI & TECH