Manufacturing method of cell fusion photoelectric sensor and working method of imaging system thereof

Abstract

The invention discloses a cell fusion photoelectric sensor and a working method of an imaging system of the cell fusion photoelectric sensor. The manufacturing method of the cell fusion photoelectricsensor comprises the following steps: rhodopsin channel protein (ChR2) extracted from a chlamydomonas reinhardtii cell eye spot is transfected into a human embryonic kidney 293 (Hek293) cell; finally,the expression of the ChR2 plasmid in the cell is observed through a fluorescence microscope, and the cell expression result is further verified through photostimulation by using a patch clamp technology; the Hek293 cell expressing ChR2 is used as a sensitive material of a photoelectric sensor, optical signals absorbed by a device are converted into an ionic current by the Hek293 cell, and a patch clamp technology is used as a signal acquisition and processing unit of the photoelectric sensor; and the cell is combined with patch clamp detection equipment to form the cell fusion photoelectricsensor. The biological fusion photoelectric device has the beneficial effects that the biological fusion photoelectric device can fully utilize the advantages of organisms in light perception or vision, so that a novel device with better performance than a pure artificial device is generated.

Description

technical field [0001] The invention relates to the field of photoelectric sensors, in particular to a photoelectric sensor for cell fusion and a working method of an imaging system thereof. Background technique [0002] Biological systems have undergone hundreds of millions of years of evolution and development in nature, and have advantages in structure and functional characteristics that many artificial systems cannot match. Integrating biological cells or tissues with electromechanical systems to build a biological fusion system can make full use of the structural and functional advantages of organisms, thereby creating some more complete structures and more powerful functional characteristics. At present, many organic fusion systems driven or powered by biological living cells or tissues have been reported in many top international journals. Montemagno et al. at the University of California, Los Angeles, cultivated muscle cells in a micromechanical structure, assembled...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a photoelectric sensor for cell fusion and the working method of its imaging system. For the first time, a photoelectric sensor for biological fusion is constructed using living cells as photosensitive units; it is difficult to realize the array of new photosensitive devices in the current technology. The problem of being unable to realize high-resolution imaging, the present invention constructs a single-pixel imaging system based on a single bio-fusion optoelectronic device to achieve high-resolution imaging

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