Method for determining contents of index components in bile after oral administration of emblic leafflower fruit tannin part and separating and identifying 30 chemical components

A technology of emblica and detection method, which is applied in the direction of material separation, measuring device, scientific instrument, etc., can solve the problems of separation and identification of metabolites, metabolism and excretion that have not been found, and achieve simple method, high accuracy and reproducibility Good results

Inactive Publication Date: 2020-06-16
BEIJING UNIV OF CHINESE MEDICINE
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  • Description
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Problems solved by technology

However, up to now, there are no relevant reports on the metabolism and excretion in bile of emblical tannins after oral administration
In addition, due to the small amount of metabolites, it is difficult to separate and identify metabolites, especially to separate and identify multiple metabolites at the same time

Method used

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  • Method for determining contents of index components in bile after oral administration of emblic leafflower fruit tannin part and separating and identifying 30 chemical components
  • Method for determining contents of index components in bile after oral administration of emblic leafflower fruit tannin part and separating and identifying 30 chemical components
  • Method for determining contents of index components in bile after oral administration of emblic leafflower fruit tannin part and separating and identifying 30 chemical components

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Embodiment Construction

[0049] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0050] Instruments and reagents

[0051] Instruments: Waters 1525 high performance liquid chromatography (binary pump, Waters 2996 UV detector, Waters, USA); Thermo high performance liquid chromatography-mass spectrometry (LTQ-Orbitrap XL HPLC-MS, Thermo Fisher Company); 100,000 One-third electronic balance (model: Sartorious BT 25S, Beijing Sartorius Instrument Co., Ltd.); high-speed centrifuge (Shanghai Anting Scientific Instrument Factory); vortex mixer (Shanghai Huxi Analytical Instrument Factory); 100-1000 shift Liquid gun (Eppendorf); ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., model: KQ-500DE); low-temperature storage refrigerator.

[0052] Test drug: Phyllanthus emblic...

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Abstract

The invention provides a method for detecting chemical substances in bile after oral administration of an emblic leafflower fruit tannin part. The method comprises the following steps: collecting a bile sample; pretreating the bile sample; determining the contents of index components including gallic acid, corilagin and ellagic acid in bile; and simultaneously separating and identifying 6 prototype components and 24 metabolites in bile. The method has the advantages of being good in separation degree, simple, convenient, accurate, high in sensitivity, good in reproducibility, large in separation and identification number and the like, and can effectively evaluate the metabolism and excretion rules of the oral emblic leafflower fruit tannin part in bile.

Description

technical field [0001] The invention relates to a method for detecting chemical substances after oral administration of tannin parts of emblica, in particular to a method for determining the content of index components in bile after oral administration of tannin parts of emblica and a method for separating and identifying 30 chemical components. Background technique [0002] After the drug is absorbed into the body, it usually goes through the process of distribution, metabolism and excretion. Drugs are metabolized in the body to produce active or new biologically active metabolites, and some drugs themselves are inactive, and only active metabolites can be produced to exert their efficacy. If the metabolism of the active ingredient is fast and eliminated in the body quickly, it will be difficult for the curative effect of the drug to exert its due effect. Bile excretion is an important factor in the elimination time of blood components in the body, which affects the blood ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 张兰珍吴玲芳梁文仪张秋楠常子豪
Owner BEIJING UNIV OF CHINESE MEDICINE
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