Variable-temperature preservation diluent for rabbit semen and preparation method thereof

Abstract

The invention belongs to the technical field of animal breeding, and specifically relates to a variable-temperature preservation diluent for rabbit semen and a preparation method thereof. The diluentis prepared by mixing a solution A and a solution B. The solution A is prepared by dissolving 5.0-35.0 g of bis(2-hydroxyethyl)amino(trimethylol)methane, 0.5-5.0 g of citric acid and 0.01-0.05 g of kanamycin with sterilized water for injection, fixing a volume to 100 ml, and successively performing subpackaging, sealing and sterilizing at a low temperature. The solution B is a glucose injection with a concentration of 5%. The solution A and the solution B are evenly mixed according to a ratio of 1: 9 to 9: 1 so as to obtain the diluent, and the pH value of the diluent is 7.0 at a temperature of 12 DEG C. The diluent provided by the invention has the advantages that sperm preservation time reaches 96 to 120 hours in a variable-temperature environment of 5 to 20 DEG C; after is the semen ispreserved with the diluent for 72 hours, sperm motility is 0.6 or above and the conception rate of female rabbits is 80% or above. Meanwhile, the diluent is simple to prepare and store and convenientto use.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and specifically relates to a diluent for storage of rabbit semen at variable temperatures and a preparation method thereof. Background technique [0002] With the continuous development of my country's rabbit industry and the promotion of the four-same method (that is, simultaneous estrus, simultaneous mating, simultaneous calving, and simultaneous slaughter), my country has gradually changed from a big rabbit-raising country to a rabbit-raising power. However, with the continuous development of rabbit artificial insemination technology, the core of the four methods, the preservation of rabbit semen has become a bottleneck in the development of artificial insemination technology. In terms of liquid storage technology of rabbit semen at home and abroad, there are many researches and designs on storage methods at low temperature (5-8°C) or normal temperature (15-18°C), most of which ar...

AI Technical Summary

Problems solved by technology

However, with the continuous development of rabbit artificial insemination technology, the core of the four methods, the preservation of rabbit semen has become a bottleneck in the development of artificial insemination technology

There are many researches and designs on low temperature (5-8°C) or normal temperature (15-18°C) storage methods in terms of rabbit semen liquid storage technology at home and abroad, and most of them are stored for a short period of 1-2 days, which limits the liquid storage of rabbit semen However, double distilled water is an unconventional product and needs to be prepared immediately, which is time-consuming and labor-intensive, and its convenience is poor.