Novel graded targeted nanoparticles for mediating phototherapy and preparation method and application of novel graded targeted nanoparticles

Abstract

The invention discloses novel graded targeted nanoparticles for mediating phototherapy and a preparation method and application of the novel graded targeted nanoparticles. The targeted nanoparticles are of a core-shell-shell spherical structure, and is prepared by adopting a W / O / W double emulsification method; and 5-ALA and CAT are distributed inside the nanoparticles as a water phase, TPP and PLGA are taken as an oil phase to form the inner shells of the nanoparticles, and the outermost layer is coated with HA to be taken as the outer shells. The nanoparticles can actively target tumor cellsand efficiently deliver a photosensitizer 5-ALA; in addition, the nanoparticles can effectively alleviate the TME hypoxia by further targeting mitochondria in the tumor cells and generating a large amount of oxygen through CAT, and can produce a large amount of ROS and enhance the killing effect of ROS under laser irradiation, so that an enhanced anti-tumor effect is exerted.

Description

technical field [0001] The invention belongs to the technical field of nano-biomedicine, in particular, it relates to a novel graded targeting nanoparticle mediating phototherapy and its preparation method and application, in particular to a novel graded nanoparticle with polylactic acid-glycolic acid copolymer as a carrier Targeted nanoparticle, its preparation method and application for treating melanoma. Background technique [0002] Melanoma is a malignant tumor originating from melanocytes in the deep epidermis. It is mostly transformed from deteriorating single melanocytes or benign dysfunctional moles. It is highly malignant and has the highest fatality rate of skin tumors. Despite the rapid development of tumor diagnosis and treatment technology in recent years, more and more drugs and therapeutic methods have been developed for the treatment of melanoma, the morbidity and mortality of melanoma are still increasing year by year worldwide, accompanied by The trend of...

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Problems solved by technology

[0006] In view of the low yield, short half-life, and limited diffusion distance of the main toxic substance ROS in photodynamic therapy due to the lack of oxygen in the tumor microenvironment, the purpose of the present invention is to provide a novel hierarchical targeting nano- Granules and their preparation methods and applications for treating melanoma, thus solving the problem of poor curative effect of ALA-PDT on melanoma and other aggressive tumors

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