Application of mustard Dilong No.1 in preparing fruit preservative
A technology for a preservative and mustard greens is applied in the application field of preparing a fruit preservative, which can solve the problems of limited fruit preservation technology, fruit rot and the like, and achieve the effects of delaying the deterioration of fruit quality, easy operation and easy availability of materials.
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Embodiment 1
[0031] Embodiment 1 Dilong No. 1 mustard
[0032] The applicant screened and bred a new mustard variety, Dilong No. 1 mustard. This mustard variety was deposited in the China Center for Type Culture Collection on December 11, 2019, with a deposit number of CCTCC NO:P201924. During the research, it was found that this variety of mustard had a remarkable effect in fruit preservation.
Embodiment 2
[0033] Embodiment 2: the preparation of fruit fresh-keeping material
[0034] In order to obtain fresh-keeping materials, the stems and leaves of Dilong No. 1 mustard in the full-flowering stage are collected in the present invention, quick-frozen in liquid nitrogen, ground, prepared into powder, packed in ziplock bags, sealed and placed in a -80°C ultra-low temperature refrigerator for later use.
[0035] The root tissues of 24 mustard varieties at the same period (such as the full flowering stage) were harvested, quick-frozen in liquid nitrogen, ground, prepared into powder, packed in ziplock bags, sealed and placed in a -80°C ultra-low temperature refrigerator for later use.
Embodiment 3
[0036] Embodiment 3: Indoor plate inhibition assay of several pathogenic bacteria by fruit fresh-keeping substances
[0037] In order to test the effectiveness of the fresh-keeping substances on the growth inhibition of pathogenic bacteria, the present invention weighs the fresh-keeping substances stored in the ultra-low temperature refrigerator and places them in half of the two-compartment petri dish. The other half was poured into the culture medium to cultivate pathogenic bacteria, placed in a constant temperature incubator at 25°C, cultivated in the dark, observed and measured the colony diameter with the cross method. Calculate the inhibitory rate of the fresh-keeping material to the pathogenic bacteria by the following formula:
[0038]
[0039] The specific plan is as follows:
[0040] ① Preparation of test strains: Inoculate Botrytis cinerea and Peronophythoralitchii cultured for about 7 days in the center of one of the two-compartment petri dishes containing PDA ...
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