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Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method

An enzymatic hydrolysis-assisted, response surface methodology technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc.

Inactive Publication Date: 2020-07-03
TONGREN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the enzymatic-ultrasound-assisted extraction of total flavonoids from Artemisia argyi.

Method used

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  • Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method
  • Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method
  • Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Artemisia argyi is dried, oven-dried, and it is pulverized into 60 mesh granules. Weigh 10.0000g of Artemisia argyi powder, add 120mg compound enzyme (cellulase: pectinase=2:1), add 150mL acetic acid-sodium acetate buffer solution, pH value is 4.5, enzymolysis at 50°C for 45min, filter, Obtain the enzymolysis filtrate and filter residue; add 150mL of 60% ethanol to the filter residue, ultrasonically extract for 20min, and the ultrasonic power is 200W, to obtain the alcohol extract; combine the enzymolysis filtrate and the alcohol extract, use a rotary evaporator to concentrate the extract, and Dry in a vacuum oven for 24 hours, weigh and record the crude extract of total flavonoids from Artemisia argyi leaves. The yield of total flavonoids from Artemisia argyi leaves was determined by ultraviolet-visible spectrophotometry to be 12.79%.

Embodiment 2

[0069] Artemisia argyi is dried, oven-dried, and it is pulverized into 60 mesh granules. Weigh 10.0000g of Artemisia argyi powder, add 140mg compound enzyme (cellulase: pectinase=2:1), obtain enzymolysis filtrate and filter residue; add 150mL acetic acid-sodium acetate buffer solution, pH value is 4.5, at 50 ℃ After 60 minutes of enzymolysis, filter, add 150mL of 60% ethanol to the filter residue, ultrasonically extract for 20min, and the ultrasonic power is (200W), to obtain the alcohol extract; combine the enzymolysis filtrate and the alcohol extract, and use a rotary evaporator to concentrate the extract , and dried in a vacuum oven for 24 hours, weighed and recorded the crude extract of total flavonoids from Artemisia argyi leaves. The yield of total flavonoids from Artemisia argyi leaves was determined by UV-visible spectrophotometry to be 12.30%.

Embodiment 3

[0071] Artemisia argyi is dried, oven-dried, and it is pulverized into 60 mesh granules. Weigh 10.0000g of mugwort leaves powder, add 100mg compound enzyme (cellulase: pectinase=2:1), add 150mL acetic acid-sodium acetate buffer solution, pH value is 4.5, enzymolysis at 50°C for 30min, filter, Obtain enzymolysis filtrate and filter residue; add 60% ethanol 150mL to filter residue, ultrasonic extraction 20min, ultrasonic power is (200W), obtain alcohol extract; Combine enzymolysis filtrate and alcohol extract, and use vacuum oven to dry, The drying time was 24 hours, and the crude extract of total flavonoids from Artemisia argyi leaves was weighed and recorded. The yield of total flavonoids in Artemisia argyi leaves was determined to be 12.25% by ultraviolet-visible spectrophotometry

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Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, and in particular to a method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by a response surface method. The method comprises the following steps: (1) performing raw material treatment: drying folium artemisiae argyi, and performing crushing to obtain folium artemisiae argyi powder; (2) performing enzymolysis: adding enzymes and a buffer solution into the folium artemisiae argyi powder, performing enzymolysis by using enzymolysis process conditions optimized by the response surface method, and then performing filtering to obtain filter residues and an enzymolysis filtrate; (3) performing extraction: adding ethanol into the filter residues for ultrasonic extraction to obtain an alcohol extraction solution; and (4) performing concentrating and drying. According to the invention, enzymolysis-assisted ultrasonic extraction is carried out on folium artemisiae argyi, and technological parameters such as enzyme dosage, enzymolysis pH value, enzymolysis time, enzymolysis temperature and the like in an extraction process are reasonably determined andselected, so that the yield of the folium artemisiae argyi total flavone reaches 10.99mg / g or above, and the ultrasonic extraction time is 20min. Therefore, the purpose of efficiently extracting folium artemisiae argyi total flavonoids is achieved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a method for optimizing enzymatic hydrolysis and ultrasonic-assisted extraction of total flavonoids from Artemisia argyi leaves by response surface method. Background technique [0002] Artemisia argyi Lévl. Et Vant is the dried leaf of the Compositae plant Artemisia argyi Lévl. Et Vant, which has the effects of warming the meridian to stop bleeding, dispelling cold and relieving pain, dispelling dampness and relieving itching. Artemisia argyi contains components such as volatile oil, sesquiterpenoids and flavonoids, among which flavonoids are the main active substances. Pharmacological studies have shown that flavonoids in Artemisia argyi leaves have the functions of scavenging free radicals, anti-oxidation, anti-aging, anti-cancer and anti-cancer. [0003] The dense structure of plant cells composed of cellulose, pectin and other components hind...

Claims

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Application Information

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IPC IPC(8): A61K36/282A61K127/00
CPCA61K36/282A61K2236/19A61K2236/333A61K2236/51
Inventor 宁娜韩建军
Owner TONGREN POLYTECHNIC COLLEGE
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