Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method

An enzymatic hydrolysis-assisted, response surface methodology technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc.

Inactive Publication Date: 2020-07-03
TONGREN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the enzymatic-ultrasound-a

Method used

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  • Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method
  • Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method
  • Method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by response surface method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0066] Example 1

[0067] The leaves are dried, dried, and pulverized into 60-mesh granules. Weigh 10.0000g of mugwort powder, add 120mg of compound enzyme (cellulase: pectinase = 2:1), add 150mL of acetic acid-sodium acetate buffer, pH 4.5, after enzymatic hydrolysis at 50 ° C for 45min, filter, Obtain enzymatic hydrolysis filtrate and filter residue; add 150 mL of 60% ethanol to the filter residue, ultrasonically extract for 20 min, and the ultrasonic power is 200 W to obtain an alcohol extract; combine the enzymatic hydrolysis filtrate and the alcohol extract, use a rotary evaporator to concentrate the extract, and Dry in a vacuum drying oven for 24h, weigh and record the crude extract of total flavonoids from the leaves of Artemisia argyi. The yield of total flavonoids in Artemisia argyi was 12.79% by UV-Vis spectrophotometry.

Example Embodiment

[0068] Example 2

[0069] The leaves are dried, dried, and pulverized into 60-mesh granules. Weigh 10.0000 g of mugwort powder, add 140 mg of compound enzyme (cellulase: pectinase = 2:1) to obtain enzymatic hydrolysis filtrate and filter residue; add 150 mL of acetic acid-sodium acetate buffer, pH 4.5, at 50 ° C After enzymatic hydrolysis for 60 min, filter, add 150 mL of 60% ethanol to the filter residue, ultrasonically extract for 20 min, and the ultrasonic power is (200W) to obtain an alcohol extract; combine the enzymatic hydrolysis filtrate and the alcohol extract, and use a rotary evaporator to concentrate the extract , and dried in a vacuum drying oven, the drying time was 24h, and the crude extract of total flavonoids from the leaves of Artemisia argyi was weighed and recorded. The yield of total flavonoids in Artemisia argyi was 12.30% by UV-Vis spectrophotometry.

Example Embodiment

[0070] Example 3

[0071] The leaves are dried, dried, and pulverized into 60-mesh granules. Weigh 10.0000 g of mugwort powder, add 100 mg of compound enzyme (cellulase: pectinase = 2:1), add 150 mL of acetic acid-sodium acetate buffer, pH value is 4.5, after enzymatic hydrolysis at 50 ° C for 30 min, filter, Obtain enzymatic hydrolysis filtrate and filter residue; add 150 mL of 60% ethanol to the filter residue, ultrasonically extract for 20 minutes, and the ultrasonic power is (200W) to obtain an alcohol extract; combine the enzymolysis filtrate and the alcohol extract, and dry in a vacuum drying oven, The drying time was 24h, and the crude extract of total flavonoids from the leaves of Artemisia argyi was weighed and recorded. The yield of total flavonoids in Artemisia argyi was 12.25% by UV-Vis spectrophotometry

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Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, and in particular to a method for optimizing enzymolysis ultrasonic assisted extraction of folium artemisiae argyi total flavone by a response surface method. The method comprises the following steps: (1) performing raw material treatment: drying folium artemisiae argyi, and performing crushing to obtain folium artemisiae argyi powder; (2) performing enzymolysis: adding enzymes and a buffer solution into the folium artemisiae argyi powder, performing enzymolysis by using enzymolysis process conditions optimized by the response surface method, and then performing filtering to obtain filter residues and an enzymolysis filtrate; (3) performing extraction: adding ethanol into the filter residues for ultrasonic extraction to obtain an alcohol extraction solution; and (4) performing concentrating and drying. According to the invention, enzymolysis-assisted ultrasonic extraction is carried out on folium artemisiae argyi, and technological parameters such as enzyme dosage, enzymolysis pH value, enzymolysis time, enzymolysis temperature and the like in an extraction process are reasonably determined andselected, so that the yield of the folium artemisiae argyi total flavone reaches 10.99mg/g or above, and the ultrasonic extraction time is 20min. Therefore, the purpose of efficiently extracting folium artemisiae argyi total flavonoids is achieved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a method for optimizing enzymatic hydrolysis and ultrasonic-assisted extraction of total flavonoids from Artemisia argyi leaves by response surface method. Background technique [0002] Artemisia argyi Lévl. Et Vant is the dried leaf of the Compositae plant Artemisia argyi Lévl. Et Vant, which has the effects of warming the meridian to stop bleeding, dispelling cold and relieving pain, dispelling dampness and relieving itching. Artemisia argyi contains components such as volatile oil, sesquiterpenoids and flavonoids, among which flavonoids are the main active substances. Pharmacological studies have shown that flavonoids in Artemisia argyi leaves have the functions of scavenging free radicals, anti-oxidation, anti-aging, anti-cancer and anti-cancer. [0003] The dense structure of plant cells composed of cellulose, pectin and other components hind...

Claims

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Application Information

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IPC IPC(8): A61K36/282A61K127/00
CPCA61K36/282A61K2236/19A61K2236/333A61K2236/51
Inventor 宁娜韩建军
Owner TONGREN POLYTECHNIC COLLEGE
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