Immobilization method for improving activity of microorganisms in well cementation cement paste

Abstract

The invention belongs to the technical field of bioengineering and oil and gas exploitation engineering, and particularly relates to an immobilization method for improving activity of microorganisms in well cementation cement paste, which mainly comprises the following steps: S1, selecting target microorganisms for expanding culture treatment, and taking bacterial liquid after expanding culture for later use; S2, taking a certain amount of porous ceramic particles, flatly laying the porous ceramic particles on a flat plate, uniformly spraying a certain amount of nutrient solution to the surfaces of the porous ceramic particles, and performing standing for 24 hours for later use; S3, preparing target porous ceramic; S4, preparing a microorganism immobilized product; and S5, preparing the microbial well cementation cement paste. The invention discloses the immobilization method for improving the activity of microorganisms in the well cementation cement paste. The microorganisms are immobilized in a porous material in this way, and then the microorganism well cementation cement paste is prepared. The activity of microorganisms in cement paste can be prolonged, porous ceramic particlesand the well cementation cement paste can be solidified into a cementation body with certain impermeability and mechanical properties through the mineralization effect of the microorganisms, and theservice life of the well cementation cement paste can be greatly prolonged.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and oil and gas exploitation engineering, and in particular relates to a method for immobilizing microorganisms in well cementing slurry. Background technique [0002] Micro-cracks in the cement sheath due to structural cracking will lead to the failure of the mechanical integrity and hydraulic sealing of the cement sheath, and in severe cases, the oil and gas well will be scrapped. With the maturity of cement-based crack microbial repair technology, it has broad application prospects in the field of cement sheath micro-crack repair. However, the current application of microbial self-healing technology in cementing slurry also faces some challenges, such as the strong alkaline environment of the cementing slurry itself, the extreme environment that is not conducive to the growth and reproduction of microorganisms downhole, the preparation of the cement slurry requires stirring, and the ceme...

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Problems solved by technology

However, the current application of microbial self-healing technology in cementing slurry also faces some challenges, such as the strong alkaline environment of the cementing slurry itself, the extreme environment that is not conducive to the growth and reproduction of microorganisms downhole, the preparation of the cement slurry requires stirring, and the cement slurry Hydration leads to constant changes in the internal pores, which will inevitably lead to the death of a large number of microorganisms. At the same time, the direct addition of microorganisms and nutrients to the cement slurry will have an adverse effect on the mechanical strength of the cement cement, which seriously restricts the mineralization of microorganisms. repair application

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