Method for enhancing contrast of microscopic CT plant sample

Abstract

The invention discloses a method for enhancing contrast of a microscopic CT plant sample. The method comprises the following steps: 1) dissolving phosphotungstic acid in an FAA stationary liquid to obtain a phosphotungstic acid solution; 2) soaking a plant part to be detected in the phosphotungstic acid solution for fixing and dyeing treatment; and (3) sequentially carrying out dehydration and carbon dioxide critical point drying on the plant part fixed and dyed in the step (2) so as to enhance the contrast ratio of the microscopic CT of the plant sample. Microscopic CT scanning (Skyscan1172,Brook Company) is performed on the sample subjected to carbon dioxide critical point drying, the scanning voltage can be 41V-59V, the resolution can be 0.56-1.76 mu m, a cross section picture is obtained through NRecon software reconstruction after the sample is scanned, the contrast ratio of plant samples at different parts is greatly improved, and the contrast ratio of parts rich in cytoplasm and protein, such as magnolia flower buds, is more remarkably improved.

Description

technical field [0001] The invention relates to a method for enhancing the contrast of micro-CT plant samples, which belongs to the field of biotechnology. Background technique [0002] Micro-CT technology has been born for more than 40 years, and it has played a huge role in medicine, and has many applications in geology and materials science. In the late 1990s, CT began to be used in plant research to study root structure, morphological development, and plant fossils, but it is rarely found in softer tissues such as young organs, tissues with low or uniform density. Research. [0003] Micro-CT imaging requires X-rays, while X-ray contrast imaging relies on the density, thickness, and atomic number of the sample (Lusic and Grinstaff, 2013). Since plant tissues are mainly composed of light elements, the X-ray absorption rate is low (about 20-40%), and the imaging effect of samples with an absorption rate of about 65-90% is the best. In addition, young plant tissues have n...

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Problems solved by technology

In the late 1990s, CT began to be used in plant research to study root structure, morphological development, and plant fossils, but it is rarely found in softer tissues such as young organs, tissues with low or uniform density. Research

These lead to no significant difference in ray transmittance, and it is very difficult to distinguish the edges of sample structures with similar densities. The reconstructed image has very little contrast and high noise (Rousseau et al., 2015)

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