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Application of capsaicin and colistin in the preparation of drugs for inhibiting Acinetobacter baumannii infection

A technology of Acinetobacter baumannii and colistin, applied in the field of biomedicine

Active Publication Date: 2022-08-05
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, what is worrying is that with the increase in frequency and dose of its use, colistin-resistant (heterogeneous drug-resistant) strains have been detected one after another

Method used

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  • Application of capsaicin and colistin in the preparation of drugs for inhibiting Acinetobacter baumannii infection
  • Application of capsaicin and colistin in the preparation of drugs for inhibiting Acinetobacter baumannii infection
  • Application of capsaicin and colistin in the preparation of drugs for inhibiting Acinetobacter baumannii infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Checkerboard test detects the synergistic effect of capsaicin and colistin

[0023] The clinical isolates of Acinetobacter baumannii Ab13, Acinetobacter baumannii Ab156 and standard strain Acinetobacter baumannii ATCC19606 were induced drug resistance, and the resulting drug-resistant strains were named: Acinetobacter baumannii Ab13- R, Acinetobacter baumannii Ab156-R and Acinetobacter baumannii ATCC19606-R. Among them, Ab13, Ab156, Ab13-R, Ab156-R and ATCC19606-R were deposited in the Laboratory of Pathogenic Microbiology, Yangzhou University. Capsaicin solvent is DMSO, colistin solvent is ddH 2 O, after configuring into mother liquor, dilute to required concentration (27g / 1000mL distilled water) with MH broth medium.

[0024] Resistant and non-resistant Acinetobacter baumannii strains were first treated with capsaicin at concentrations of 256mg / L, 128mg / L, 64mg / L, 32mg / L and 16mg / L alone, and the results showed that capsaicin alone was effective for all...

Embodiment 2

[0028] Example 2 Time sterilization curve detection of the synergistic effect of capsaicin and colistin

[0029] In order to further prove the synergistic effect of capsaicin and colistin, a time sterilization curve test was carried out on the above-mentioned three strains of colistin-resistant Acinetobacter baumannii. respectively to 5×10 9 CFU / mL of Ab13-R and 5×10 6 Different drugs were added to the Ab156-R and ATCC19606-R inoculum of CFU / mL, cultured at 37°C on a shaker, and samples were taken every 2h for plate count. In this test, the concentration of colistin was the clinical sensitive critical value: 2 mg / L, the concentration of capsaicin was 256 mg / L for Ab13-R, and 32 mg / L for Ab156-R and ATCC19606-R. see the results figure 1 , it can be seen that there was no significant difference in bacterial growth between the single drug group and the control group within 12h; however, when capsaicin was combined with colistin, more than 50% of the bacteria were killed within...

Embodiment 3

[0030] Example 3 Synergistic effect of capsaicin and colistin in vivo

[0031] First use 2×10 7 CFU / mouse A13-R strain suspension was intraperitoneally injected into mice, and no death was seen after 24 hours, indicating that the isolated and induced colistin-resistant strain A13-R was less virulent. Then mice were intraperitoneally injected with A13-R strain suspension, and the control group was injected with sterile PBS; the drug treatment group received treatment 24 hours after the mice were infected, the colistin group received 5 mg / kg colistin; the capsaicin group received 200 μmol / L Treatment with capsaicin solution; in the combined group, 1 mg / kg colistin and 100 μmol / L capsaicin solution were injected in two doses with an interval of 2 h. After the treatment continued for 3 days, blood was collected from the eyeball and the lungs, kidneys, spleen and liver were collected to detect the load of bacteria. The results are shown in Table 2. figure 2 and image 3 , it ...

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Abstract

The invention provides the application of capsaicin and colistin in preparing a medicine for inhibiting Acinetobacter baumannii infection, belonging to the technical field of biomedicine. The Acinetobacter baumannii includes colistin-resistant Acinetobacter baumannii; Capsaicin can synergize with colistin to play an anti-Acinetobacter baumannii effect, thereby treating infections caused by colistin-resistant Acinetobacter baumannii. When capsaicin was used in combination with colistin, more than 50% of colistin-resistant Acinetobacter baumannii were killed within 12 hours, and the CFU per ml was reduced by more than 2log10 after 12 hours, proving the synergy between capsaicin and colistin effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of capsaicin and colistin in the preparation of medicines for inhibiting Acinetobacter baumannii infection. Background technique [0002] Acinetobacter baumannii (Ab) is an obligate, aerobic, non-fermenting, Gram-negative bacillus that is highly susceptible to infection in patients with severe underlying diseases or autoimmune compromise, causing ventilator-associated pneumonia (VAP). ), bacteremia, skin and soft tissue infections, endocarditis, urinary tract infections and meningitis. Therefore, it is particularly important to find new strategies to treat Acinetobacter baumannii infection. [0003] Due to the scarcity of effective therapeutic drugs and newly developed antibiotics, some traditional antibiotics such as polymyxins (colistin, polymyxin B) that have been abandoned have been forced to be reintroduced to treat multidrug-resistant Gram-negative bact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/12A61K31/165A61P31/04
CPCA61K38/12A61K31/165A61P31/04A61K2300/00
Inventor 郭停停李梦影郑文浩孙晓利郇长超李国才
Owner YANGZHOU UNIV
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