Kit and detection system

Abstract

The embodiment of the invention relates to the technical field of chemical analysis, and particularly relates to a kit and a detection system. The kit comprises a shell, a reagent, a magnetic card anda stirrer. The reagent comprises an antiserum, a buffer solution and a quality control product, wherein the antiserum includes a first phosphate buffer solution and latex particles coupled with a goat anti-human serum amyloid protein A polyclonal antibody; the buffer solution includes a second phosphate buffer solution, sodium chloride and sodium azide; the quality control product includes a solution containing serum amyloid protein A; a mass ratio of the first phosphate buffer solution to the latex particles coupled with the goat anti-human serum amyloid protein A polyclonal antibody is (3-10): 1; and the mass ratio of the second phosphate buffer solution to sodium chloride is 1: (1-2), the mass ratio of the second phosphate buffer solution to sodium azide is (9-12): 1, and a concentration of serum amyloid protein A in the quality control product is 5-300 mg / L. When the kit is used for detecting the content of the serum amyloid protein A, detection time is short, and an operation process is simple.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of chemical analysis, in particular to a kit and a detection system. Background technique [0002] Serum amyloid A (serum amyloid A, SAA), the precursor of tissue amyloid A, is a polymorphic protein encoded by multiple genes and belongs to the acute phase response protein [1]. According to the expression, SAA can be divided into two types: constitutive SAA (constitutive SAA, C-SAA) and acute SAA (acute SAA, A-SAA). Under normal circumstances, SAA in the human body is mainly derived from C-SAA in liver cells, accounting for more than 90% of the total SAA. When the body is stimulated, such as inflammation, infection, tumor, etc., the body releases pro-inflammatory factors (such as interleukin, tumor necrosis factor-α, etc.) Rapidly rises 100-1000 times within 5-6 hours and falls rapidly during the recovery period of the disease. Based on this, detecting the content of serum amyloid...

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Problems solved by technology

[0004] However, in the process of realizing the embodiment of the present invention, the inventors of the present invention found that the detection sensitivity of the enzyme-linked immunosorbent assay method is low, and the operation steps are complicated, and a standard curve needs to be prepared at the same time for each detection, which is time-consuming and expensive. laborious

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