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Foot and mouth disease virus-like particle antigen, vaccine composition, preparation method and application

A technology of foot-and-mouth disease virus and particle antigen, applied in the direction of virus antigen components, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of weak immunogenicity of vaccines, and achieve the level of no biosafety risk and antibody production High, rapid antibody production

Active Publication Date: 2020-07-21
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defect that the immunogenicity of the O-type foot-and-mouth disease virus inactivated vaccine in the prior art is not strong, the present invention provides a kind of O-type foot-and-mouth disease virus-like particle antigen, wherein, the O-type foot-and-mouth disease virus-like particle antigen is CATHAY type Type O foot-and-mouth disease virus-like particle antigen, the CATHAY type O foot-and-mouth disease virus-like particle antigen is assembled from CATHAY type O foot-and-mouth disease virus VP0, VP3, VP1 antigenic protein

Method used

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  • Foot and mouth disease virus-like particle antigen, vaccine composition, preparation method and application
  • Foot and mouth disease virus-like particle antigen, vaccine composition, preparation method and application
  • Foot and mouth disease virus-like particle antigen, vaccine composition, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1CATHAY type O type foot-and-mouth disease virus-like particle

[0086] The bacterial cells expressing the antigenic protein were resuspended and detected by SDS-PAGE electrophoresis. At this time, the expression levels of the three serially expressed proteins in the supernatant were all about 20%. The purified protein was subjected to SDS-PAGE electrophoresis, which showed that the target protein was purified and enriched.

[0087] Negative staining with phosphotungstic acid and electron microscope observation showed that the CATHAY-type O-type FMD protein formed virus-like particles, and the formed virus-like particles were plump, with high assembly efficiency and no aggregation. After placing the foot-and-mouth disease virus-like particles at 4°C for 3 months, negative staining with phosphotungstic acid and electron microscope observation showed that the virus-like particles were still full without aggregation. It shows that the foot-and-mouth disease pro...

Embodiment 2

[0088] The preparation of embodiment 2CATHAY type O type foot-and-mouth disease virus-like particle vaccine composition

[0089] The specific ratio of each component in the prepared vaccine is shown in Table 1.

[0090] Table 1 CATHAY type O-type foot-and-mouth disease virus-like particle vaccine composition composition ratio

[0091] Vaccine 1 Vaccine 2 Vaccine 3 FMD antigen (μg / ml) 160 200 240 ISA 206 Adjuvant (V / V%) 50％ 50％ 50％

Embodiment 3

[0092] Example 3 The immunogenicity test of the vaccine composition containing CATHAY type O-type foot-and-mouth disease virus-like particle antigen

[0093] Select 20 healthy susceptible pigs with a weight of about 40kg and are negative for type O foot-and-mouth disease virus antigen and antibody, and divide them into 4 groups randomly, 5 pigs in each group. Groups 1-3 are vaccine 1, vaccine 2, and vaccine 3 immunization groups prepared in Example 2 of the present invention, respectively, and group 4 is a blank control group. The way of immunization in the immunized group was intramuscular injection of 2 ml in the neck, and the control group was immunized with the same amount of PBS.

[0094] Antibody titer results showed that the antibody titer of all pigs before the vaccine immunization was negative, and all pigs could reach 1:128 or more on the 14th day after the first immunization. The antibody of pigs in the blank control group was negative and there was no change. The...

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Abstract

The invention provides an O-type foot and mouth disease virus-like particle antigen. The O-type foot and mouth disease virus-like particle antigen is a CATHAY-type O-type foot and mouth disease virus-like particle antigen and the CATHAY-type O-type foot and mouth disease virus-like particle antigen is composed from the composition of CATHAY-type O-type foot and mouth disease virus VP0, VP3 and VP1antigen proteins. The O-type foot and mouth disease virus-like particle antigen has good immunogenicity, a prepared vaccine can realize primary immunization, complete protection against O-type foot and mouth disease virus can be realized on the 14th day after immunization, the titer of a generated antibody is higher than the titer of a commercial inactivated vaccine, and the protective immune period can last for at least 133 days. The invention further relates to a prepared vaccine composition and a preparation method thereof and application.

Description

technical field [0001] The present invention belongs to viral antigen and the pharmaceutical preparation of the antigen. Specifically, the present invention relates to a foot-and-mouth disease virus-like particle antigen, a vaccine composition prepared by it, a preparation method and application thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease that can spread quickly and over long distances. Significant economic losses worldwide. Animals affected by FMD include cattle, sheep and pigs. The causative agent is foot-and-mouth disease virus (FMDV), an aphthous sore virus of the picornavirus family. The virus is divided into seven serotypes (A, O, C, Asia 1, SAT1, SAT2 and SAT3), of which the O-type foot-and-mouth disease virus is the most prevalent. According to genetic classification, there are currently three types of Topotype O-type FMD viruses prevalent in China, which belong to CATHAY type (Chinese type), M...

Claims

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Application Information

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IPC IPC(8): C07K14/09C12N15/42C12N15/70A61K39/135A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2770/32123C12N2770/32134A61K2039/5258A61K2039/552Y02A50/30Y02A40/70
Inventor 田克恭肖燕逄文强孙进忠张许科
Owner PU LIKE BIO ENG
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