Amplifying and sequencing primer for subtyping and drug resistance detection of helicobacter pylori, and reagent kit
A technology of Helicobacter pylori and sequencing primers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., which can solve the problems of multiple mutation types and incomplete detection of Helicobacter pylori, and avoid Complicated and difficult, reducing manpower and material resources, and the effect of multiple experimental information
Pending Publication Date: 2020-07-28
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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Abstract
The invention discloses an amplifying and sequencing primer for subtyping and drug resistance detection of helicobacter pylori, and a reagent kit. The reagent kit comprises reaction liquid A for PCR amplification, a reaction tube for coating primer pairs, and primers H1, H2, H3, H4, H5, H6, H7, H8, H9, H10 and H11 for sequencing. The amplifying and sequencing primer for subtyping and drug resistance detection of helicobacter pylori, and the reagent kit disclosed by the invention can amplify all 11 objective sequences of the helicobacter pylori under the same PCR reaction condition at one time,and then the sequencing primers are used for performing sequencing according to a set procedure. A PCR reaction system has fluorescent dye, an amplification result can be judged directly through theelevation position of a fluorescent value, and inconvenience and pollution caused by electrophoresis can be avoided. The primers are directly coated in the reaction tube, so that tedious configurationduring primer use can be avoided. The reagent kit can be used for detecting virulence subtypes and drug resistance genes of the helicobacter pylori. The amplifying and sequencing primer and the reagent kit disclosed by the invention can be used for detecting clinical biopsy specimens and paraffin-embedded specimens and can provide bacterium subtypes and drug resistance information for helicobacter pylori treatment.
Application Domain
Microbiological testing/measurementDNA/RNA fragmentation
Technology Topic
FluorescenceVirulence +10
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