New application of minor radix buplenri granules to prevention and treatment of syncytial viruses and adenoviruses

A technology of syncytial virus and adenovirus, applied in the field of Chinese patent medicine, can solve the problems that have not yet been related to the application of Xiao Chai Hu granules

Pending Publication Date: 2020-07-31
GUANGZHOU BAIYUSN GUANGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no application of Xiaochaihu granules in inhibiting syncytial virus, adenovirus and the free radical damage caused by them

Method used

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  • New application of minor radix buplenri granules to prevention and treatment of syncytial viruses and adenoviruses
  • New application of minor radix buplenri granules to prevention and treatment of syncytial viruses and adenoviruses
  • New application of minor radix buplenri granules to prevention and treatment of syncytial viruses and adenoviruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: In vitro antiviral inhibition test of Xiaochaihu granules

[0027] 1. Experimental materials:

[0028] 1.1 Test drug: Xiaochaihu Granules (sugar-free, Guangzhou Baiyunshan Guanghua Pharmaceutical Co., Ltd.).

[0029] 1.2 Control drug: ribavirin.

[0030] 1.3 Virus strains: syncytial virus (RSV, Long strain), adenovirus (ADV3, ADV4, ADV5, ADV6, ADV7, ADV11, ADV21). The virus was continuously passaged twice with Hela cell monolayer, and the cell culture medium was harvested and stored in a -80°C refrigerator for later use.

[0031] 1.4 Cell model: Hep-2 cells.

[0032] 2. Experimental method:

[0033] 2.1 Determination of virus virulence

[0034] 100 μl of 10-concentration virus solutions obtained by 10-fold serial dilution were inoculated into 96-well plates covered with a single layer of Hep-2 cells in sequence in 8 replicate wells for each concentration, and a cell control was set at the same time. 37°C, 5% CO 2Cultured in the virus incubator, the cyt...

Embodiment 2

[0043] Example 2: In vivo experiment of Xiaochaihu granules on syncytial virus and adenovirus infected mouse pneumonia model

[0044] 1. Experimental materials:

[0045] 1.1 Test drug: Xiaochaihu Granules (sugar-free, Guangzhou Baiyunshan Guanghua Pharmaceutical Co., Ltd.).

[0046] 1.2 Control drug: ribavirin.

[0047] 1.3 Viruses: syncytial virus (RSV, Long strain), adenovirus (ADV3 type).

[0048] 2. Experimental animals: SPF grade Kunming mice, 18-20g, 120 males, provided by Guangdong Medical Experimental Animal Center, certificate number SYXK (Guangdong) 2019-0185. Start the experiment one week after the experimental animals adapt to the new environment, and take appropriate measures to reduce the damage to the animals during the experiment.

[0049] 3. Experimental method:

[0050] 3.1 Anti-syncytial virus infection mouse pneumonia model

[0051] The mice were randomly divided into a blank control group, an RSV model group, a ribavirin group, a low-dose Xiaochaihu g...

Embodiment 3

[0065] Example 3: Xiaochaihu Granules Eliminate Excessive Free Radicals in Mouse Models of Pneumonia Infected by Syncytial Virus and Adenovirus

[0066] 1. Experimental materials:

[0067] 1.1 Test drug: Xiaochaihu Granules (sugar-free, Guangzhou Baiyunshan Guanghua Pharmaceutical Co., Ltd.).

[0068] 1.2 Control drug: ribavirin.

[0069] 1.3 Viruses: syncytial virus (RSV, Long strain), adenovirus (ADV3 type).

[0070] 1.4. Experimental animals: SPF grade Kunming mice, 18-20g, 120 males, provided by Guangdong Medical Experimental Animal Center, certificate number SYXK (Guangdong) 2019-0185. Start the experiment one week after the experimental animals adapt to the new environment, and take appropriate measures to reduce the damage to the animals during the experiment.

[0071] 2. Experimental method:

[0072] 2.1 Anti-syncytial virus infection mouse pneumonia model

[0073] The mice were randomly divided into a blank control group, an RSV model group, a ribavirin group, a ...

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Abstract

The invention relates to an application of minor radix buplenri granules in preparation of a medicine for preventing and treating diseases caused by infection of respiratory tract viruses. It is discovered that the minor radix buplenri granules have good in-vitro antiviral (syncytial viruses and adenoviruses) activity, can improve lung injury caused by the syncytial viruses and the adenoviruses, obviously relieve respiratory tract peroxidation injury caused by the viruses, obviously enhance the free radical scavenging capacity (obviously regulating and controlling CAT, SOD, GSH-px, MDA, NO, MPO, TNF-a, IL-6, ROS, superoxide anion resistance and the like) of the body, and present dose-effect relationship characteristics. Namely, the new application of the minor radix buplenri granules in inhibiting the syncytial viruses, the adenoviruses and free radical injuries caused by the syncytial viruses and the adenoviruses is found.

Description

technical field [0001] The invention belongs to the field of Chinese patent medicines, and relates to a new application of a traditional Chinese medicine composition-Xiabupleurum granules in the prevention and treatment of syncytial virus and adenovirus. Background technique [0002] The main symptoms of respiratory tract infection are fever, cough, nasal congestion, etc. The disease is mainly caused by bacterial and viral infections, among which viral infections account for more than 90%, and among viruses, syncytial virus and adenovirus are particularly common. Respiratory syncytial virus is an RNA virus belonging to the Paramyxoviridae family. In the past ten years, syncytial virus pneumonia and bronchiolitis accounted for the first viral pneumonia in infants and young children in my country, and their symptoms were almost indistinguishable clinically from parainfluenza virus pneumonia, mild influenza virus pneumonia and mild adenovirus pneumonia. Adenovirus (adenovirus)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9068A61K9/16A61P11/00A61P31/14A61P31/20
CPCA61K9/16A61K36/233A61K36/344A61K36/484A61K36/539A61K36/725A61K36/8888A61K36/9068A61P11/00A61P31/14A61P31/20A61K2300/00
Inventor 刘宏黄伟陈祥云毕聪郑如文江志强张俊华何逸禧杜海泳
Owner GUANGZHOU BAIYUSN GUANGHUA PHARMA
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