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Efficient extraction method of loach myofibrillar protein

A myofibril and extraction method technology, which is applied in the field of efficient extraction of loach myofibril protein, can solve the problems of low extraction rate and protein instability, and achieve the effects of high extraction efficiency, high purity, and shortened extraction time

Inactive Publication Date: 2020-07-31
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing method for extracting loach myofibrillar protein has disadvantages such as low extraction rate and unstable protein, so it needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The high-efficiency extraction method of loach myofibrillar protein is to use loach as raw material, remove the tail, put in cold water to let blood, then remove the head and viscera, wash and collect the meat. The extraction steps are as follows:

[0021] (1) After washing the loach meat with tap water three times, add 4 times the volume of cold (4°C) 50mmol / L phosphate buffer solution (0.1mol / L NaCl, 1mmol / L EDTA, 2mmol / L MgCl 2 , pH value is 7.0), homogenize in a homogenizer at high speed for 3min (8000rpm, homogenize for 30s, stop for 30s). at 8.96 x 10 7 Under Pa high-pressure micro-jet treatment, the obtained homogenate was centrifuged at 8000r / min and 4°C for 15 minutes, and then the precipitate was collected, and the above steps were repeated for a total of 3 extractions;

[0022] (2) Then add 4 times the volume of cold (4°C) 50mmol / L phosphate buffer solution (0.6mol / LNaCl, pH value 7.0) to the precipitate, and homogenize at a high speed for 30s (8000rpm) in ...

Embodiment 2

[0025] The high-efficiency extraction method of loach myofibril protein is to use loach as raw material, remove the tail and put in cold water to let blood, then remove the head and viscera, wash and collect the meat. The extraction steps are as follows:

[0026] (1) After washing the loach meat with tap water three times, add 4 times the volume of cold (4°C) 50mmol / L phosphate buffer solution (0.1mol / L NaCl, 1mmol / L EDTA, 2mmol / L MgCl 2 , pH value is 7.0), homogenize in a homogenizer at high speed for 3min (8000rpm, homogenize for 30s, stop for 30s). at 6.89×10 7 Under Pa high-pressure micro-jet treatment, the obtained homogenate was centrifuged at 8500r / min and 4°C for 20 minutes, and then the precipitate was collected, and the above steps were repeated for a total of 3 extractions;

[0027] (2) Then add 6 times the volume of cold (4°C) 50mmol / L phosphate buffer solution (0.6mol / LNaCl, pH value 7.0) to the precipitate, and homogenize at a high speed for 30s (8000rpm) in a ...

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Abstract

The invention relates to an efficient extraction method of loach myofibrillar protein, and belongs to the technical field of aquatic product processing. The method comprises the following steps: taking meat from fresh and alive loaches, and washing the meat with tap water for at least three times; adding a cold phosphate buffer solution with low ionic strength into the loach meat, performing homogenizing at a high speed, performing treating under a high-pressure microjet technology, centrifuging a feed liquid, taking precipitate, and repeating the above steps for at least three times; and adding a cold phosphate buffer solution with high ionic strength into the precipitate, performing homogenizing at a high speed, performing treating under the high-pressure microjet technology, extractingand concentrating a feed liquid by using a circulating ultrafiltration membrane system, finally performing centrifuging, and taking a supernatant to obtain a myofibrillar protein solution. The methodis short in extraction time and high in extraction efficiency and yield, and the obtained protein is high in purity and good in stability.

Description

technical field [0001] The invention belongs to the technical field of aquatic product processing, and in particular relates to an efficient extraction method of loach myofibril protein. Background technique [0002] my country has vast waters and rich fishery resources. Since the 1980s, the total output of aquatic products has grown rapidly, and China has become the world's largest fishery country. [0003] Loach is a small fish with high protein content and low lipid content. It is widely distributed in natural freshwater areas in various places in my country. It has rich nutritional value and unique flavor. Loach is rich in protein, which is easily digested and absorbed by the human body, and can meet the essential amino acids, minerals and other nutrients; the protein in fish meat is relatively complete in nutrition, and the myofibrillar protein has good functionality. [0004] Myofibrillar protein is a group of structural proteins with important biological functions. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/36C07K1/34C07K1/30
CPCC07K14/43504
Inventor 胡爱军于辉郑捷
Owner TIANJIN UNIV OF SCI & TECH
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