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Pseudomonas with algae-dissolving activity and its application

A kind of Pseudomonas, active technology, applied in the field of microbial algae control, can solve the problems in the initial stage, influence, few successful application cases, etc., achieve the effect of free retractable, long service life, and reduce additional nutrient load

Active Publication Date: 2022-03-15
INST OF AQUATIC LIFE ACAD SINICA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in view of the fact that its actual algicidal effect is easily affected by various biotic and abiotic factors in the water ecosystem, and the ecological safety of some microorganisms needs to be further scientifically demonstrated, the current research on algicidal bacteria is mainly focused on the isolation, identification, and dissolution of algae. In terms of the description of algae phenomenon and its algae-dissolving method and mechanism, the research on its field application method is still in its infancy, and there are few cases of its successful application

Method used

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  • Pseudomonas with algae-dissolving activity and its application
  • Pseudomonas with algae-dissolving activity and its application
  • Pseudomonas with algae-dissolving activity and its application

Examples

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Embodiment 1

[0026] Example 1: Isolation, purification and cultivation of Pseudomonas sp. THW7

[0027] 1. Isolation, purification and preservation of Pseudomonas THW7

[0028] The separation and purification process of algae-lytic bacteria is as follows: figure 1 shown. Water samples of cyanobacteria blooms were collected from Taihu Lake, filtered through a 0.8 μm filter membrane (Millipore), and the supernatant was diluted 10 times with sterile water (the dilution level depends on the specific situation), and 100 μL of each gradient dilution was evenly coated Distribute on LB solid plates, each gradient has three parallels, write the corresponding number, and incubate upside down in a biochemical incubator at 30°C for 48 hours. Select a plate with a relatively uniform distribution of colonies, pick single colonies of different forms on the LB solid plate, separate and purify twice, and obtain purely cultured strains, which are stored in a -80°C refrigerator with glycerol preservation. ...

Embodiment 2

[0040] Embodiment 2: the application of Pseudomonas THW7 in controlling cyanobacteria bloom

[0041] Take the logarithmic period (20h, OD 600nm =1.8) at room temperature for centrifugation (5000rpm, 6min), collect the bacteria, wash 2-3 times with sterile water to wash away the LB medium on the surface of the bacteria. The obtained bacteria are resuspended by adding sterile water (as small a volume as possible) to obtain a bacterial suspension for future use.

[0042] Add the supersuspension of bacteria into the sterilized sodium alginate solution with a mass concentration of 3%, stir and mix with a clean glass rod, and inject the mixture dropwise into calcium chloride with a mass concentration of 4% with a syringe solution, cross-linked for 30min. Wash the embedding pellets with sterile water for 3-5 times to wash away the calcium chloride solution on the surface, and prepare bacterial cell embedding pellets with a diameter of about 4-6 mm for later use, such as Figure 6 ...

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Abstract

The invention discloses a Pseudomonas ( Pseudomonas sp.) THW7, the preservation number is CCTCC NO:M 20191065, this strain has high algalysis efficiency, it is made into bacteria-embedded pellets and put into cyanobacteria bloom water samples, and then supplemented with a certain concentration of glucose As an energy supplement, it has a good effect of controlling cyanobacteria blooms. At the same time, the bacterial cell embedding ball has a long service life and can be used repeatedly, which is safe and environmentally friendly.

Description

technical field [0001] The invention belongs to the field of algae control by microorganisms, in particular to a pseudomonas with algae-dissolving activity and its application in controlling cyanobacteria blooms. Background technique [0002] In the past two decades, cyanobacterial blooms in freshwater bodies in China have generally shown the characteristics of high biomass, large area, high frequency, and high hazard. The cyanobacteria that form water blooms mainly include Microcystis, Dolichospermum, Aphanizomenon, Planktothrix, Nodularia, Oscillatoria, etc. Microcystis blooms are the most important type of water blooms in China, followed by long sporospores blooms and atenocystis blooms. [0003] The wanton outbreak of cyanobacteria bloom directly or indirectly affects human health, and causes the imbalance of the structure and function of the water ecosystem. It is of great theoretical and practical significance to study and develop key technologies for algae control a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/04C02F3/34C12R1/38
CPCC12N11/10C12N11/04C02F3/34C02F2305/06C12R2001/38C12N1/205
Inventor 甘南琴宋立荣陈莉婷
Owner INST OF AQUATIC LIFE ACAD SINICA
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