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Method for extracting flavonoid compounds from lamiophlomis rotata by response surface method

A response surface methodology, a unique technology, applied in pharmaceutical formulations, plant raw materials, design optimization/simulation, etc., which can solve the problem of low extraction rate of total flavonoids in the unique flavor, low extraction rate, and inability to clearly find the response value and independent variable function. Expressions etc.

Inactive Publication Date: 2020-08-07
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Conventional extraction methods for flavonoids include maceration, reflux extraction, and Soxhlet extraction, etc., which mainly have the following problems: consumption of a large amount of organic solvent, time-consuming and low extraction rate
[0004] The traditional optimization method orthogonal experiment extracts the total flavonoids in Duyiwei, the extraction rate is low, and it is impossible to clearly find the functional expression between the response value and the independent variable
It is necessary to develop a method to solve the problem of low extraction rate of total flavonoids in the traditional extraction method

Method used

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  • Method for extracting flavonoid compounds from lamiophlomis rotata by response surface method
  • Method for extracting flavonoid compounds from lamiophlomis rotata by response surface method
  • Method for extracting flavonoid compounds from lamiophlomis rotata by response surface method

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preparation example Construction

[0060] The preparation method of the rutin absorbance standard curve is as follows: take 50.0 mg rutin standard substance, dilute it to a 250mL volumetric flask with ethanol with a volume fraction of 70%, and prepare a rutin standard substance mother solution with a concentration of 0.2mg / mL. Then accurately measure 2.5, 5.0, 7.5, 10.0, 12.5mL rutin standard mother solution respectively and place them in a 25mL volumetric flask, and take 3 parallel concentrations for each gradient; use NaNO 2 -Al(NO 3 ) 3 Make a standard curve by the colorimetric method, add 0.3mL 5% NaNO to the 25mL volumetric product containing different amounts of rutin standard mother solution respectively 2 , shake well, let stand for 10min; add 0.3mL 10% Al(NO 3 ) 3 , shake well, let stand for 10min; then add 4mL 4% NaOH, shake well, dilute to 25mL with 70% ethanol, mix well, let stand for 15min. 70% ethanol was used as the blank control group. At 505nm, measure the absorbance value, and measure the...

Embodiment 1

[0070] Pulverize the Duyiwei medicinal material and pass through a 40-mesh sieve to obtain the Duyiwei powder. Weigh 2.0g of the pretreated Duyiwei powder, fix the ethanol volume fraction to 50%, ultrasonic power to 240W, extraction time to 20min, and a solid-to-liquid ratio of 1 : 20g / mL, taking soaking time 0, 2, 4, 6, 8h as experimental points respectively, investigates the influence of different soaking times on the extraction rate of total flavonoids of Duyi. Such as figure 2 As shown, with the increase of soaking time, the extraction rate of total flavonoids increased continuously. When the soaking time increased from 0h to 4h, the extraction rate of total flavonoids increased significantly, but the extraction rate decreased after 4h. Therefore, choose 4 hours as the best soaking time.

Embodiment 2

[0072] Pulverize the Duyiwei medicinal material and pass through a 40-mesh sieve to obtain the Duyiwei powder. Weigh 2.0g of the pretreated Duyiwei powder, fix the soaking time for 4h, ultrasonic power is 240W, extraction time is 20min, and the solid-liquid ratio is 1:20g / mL, taking the volume fraction of ethanol as 10%, 30%, 50%, 70%, and 90% as experimental points respectively, to investigate the influence of different volume fractions of ethanol on the extraction rate of total flavonoids of unifoliate, as image 3 As shown, with the increase of ethanol volume fraction, the extraction rate of flavonoids of Duyiwei first increased and then decreased. There is a decline. However, when the volume fraction of ethanol exceeds 70%, the extraction yield drops rapidly.

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Abstract

The invention provides a method for extracting flavonoid compounds from lamiophlomis rotata by a response surface method, which comprises the following steps: A) pulverizing lamiophlomis rotata, and screening to obtain medicinal material powder; B) adding the medicinal material powder into a 10-90% ethanol solution according to a solid-to-liquid ratio of 1: 10-1: 50g / mL, soaking for 2-8h, carryingout ultrasonic extraction for 10-30min, and filtering to obtain a flavonoid compound extracting solution; wherein the ultrasonic extraction conditions of the soaking time, the ethanol volume fractionand the solid-to-liquid ratio are optimized by adopting a response surface method. The extraction process for extracting flavone from lamiophlomis rotata by an ultrasonic method is optimized throughsingle factor and response surface method experiments, so that the optimal extraction condition is obtained. The method solves the problem of low extraction rate of the total flavonoids in the lamiophlomis rotata by a traditional method, and provides a theoretical basis for large-scale production of the flavonoids in the lamiophlomis rotata and development of flavonoid compounds in plants.

Description

technical field [0001] The invention relates to the technical field of extraction of natural products, in particular to a method for extracting flavonoids in unique flavor by response surface method. Background technique [0002] Lamiophlomis rotata (Benth.) Kudo is a unique plant from the Qinghai-Tibet Plateau. It is widely distributed in Tibet, Qinghai, Gansu and Sichuan. Its aerial part shows a wide range of pharmacological activities, such as: treatment of bruises, traumatic bleeding, yellow water disease, rheumatic arthralgia, promoting blood circulation and removing blood stasis, dispelling wind and relieving pain, etc. There are two main types of ingredients in Duyiwei: iridoid glycosides and flavonoids. There are more studies on iridoid glycosides, but less attention has been paid to flavonoids. However, the pharmacological activities of flavonoids are important, such as: antioxidant, antidepressant, antidiabetic, anti-inflammatory, antitumor and neuroprotective e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53G06F30/20G06F111/04G06F17/18
CPCA61K36/53A61K2236/333A61K2236/39G06F17/18
Inventor 崔勐孙丽影杜杨赵凤娇
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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