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Application of PGR as product for treating endometriosis, and kit for detecting PGR

An endometrial and kit technology, applied in the field of PGR, can solve problems such as unclear mechanism

Inactive Publication Date: 2020-08-07
湖南省科域生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that progesterone receptor (PGR) may play a role in endometriosis, but the specific mechanism is not clear

Method used

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  • Application of PGR as product for treating endometriosis, and kit for detecting PGR
  • Application of PGR as product for treating endometriosis, and kit for detecting PGR
  • Application of PGR as product for treating endometriosis, and kit for detecting PGR

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Low expression of PGR in endometriosis

[0068] It has been shown in the literature that the PGR gene is differentially expressed in endometriosis. In order to verify the expression of the differentially expressed gene PGR in endometriosis, we first detected 86 Ems patients (EME) and 54 non-Ems patients at the same time ( EM) endometrial tissue, analysis shows that qRT-PCR and WB results show ( figure 1 A-B), compared with the EM group, the expression of PGR in the endometrial tissue of the EME group was significantly decreased (Pfigure 1 C): The endometrial epithelial cells of the mice in the Normol group and the Sham group were tall columnar or columnar, and the number of glands was large. The glandular epithelial cells were mostly columnar, and subnuclear vacuoles and apocrine secretion could be seen. Compared with the Normal group, the ectopic endometriosis foci in the EMS group was multicystic, and the outer wall of the capsule was covered with a layer o...

Embodiment 2

[0073] Example 2: PGR inhibits TGF-β signaling pathway and regulates endometriosis

[0074] The literature suggests that the TGF-β signaling pathway is activated in endometriosis. We also verified this conclusion. In clinical tissue samples, the expression differences of TGF-β pathway-related genes TGF-β1, smad2 and Pai-1 were detected, and the results showed that ( figure 2 A), compared with the EM group, the expressions of TGF-β1, p-smad2 and Pai-1 in the endometrial tissues of the EM group were significantly increased (P0.05). We further studied the effect of PGR on the TGF-β signaling pathway, and WB detected the expression of genes related to the TGF-β signaling pathway after PGR was affected. The results showed that ( figure 2 B) Compared with oe-NC, the expression levels of TGF-β1, p-smad2 and Pai-1 in the cells of oe-PGR group were significantly decreased (P0.05). And then to study whether PGR TGF-β signaling pathway affects endometrial cell function. We first use...

Embodiment 3

[0076] Example 3: TGIF1 competitively binds to smad2 to inhibit TGF-β pathway and relieve endometriosis

[0077] Studies have shown that TGIF1 competitively binds to smad2 to inhibit the TGF-β signaling pathway [PMID:20622901]. We first verified the expression difference of TGIF1 in clinical tissue samples, and the qRT-PCR results showed that ( image 3 A) Compared with the EM group, the expression of TGIF1 mRNA in the endometrial tissue of the EME group had no significant change (P>0.05). The WB results showed (Figure 3B), compared with the EM group, the expression of TGIF1 in the endometrial tissue of the EME group was significantly decreased (P image 3 C), compared with the oe-NC group, the expression levels of Smad2 and Pai-1 proteins in the cells of the oe-TGIF1 group were significantly decreased (P image 3 D-E), after overexpressing TGIF1, the invasion and proliferation ability of hESC were significantly improved, and the difference was statistically significant (P image...

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Abstract

The invention discloses a protein marker for treating endometriosis. Specifically, the protein marker is PGR. The invention also discloses application of a PGR gene in the preparation of a product fortreating endometriosis, a pharmaceutical composition for treating endometriosis and a kit for evaluating the expression level of the PGR gene. The pharmaceutical composition disclosed by the invention has a relatively good inhibition effect on endometriosis, and has important reference and practical significance in endometriosis treatment. The kit provided by the invention can be used as one of evaluation means of the PGR gene expression level, and has good measurement and guidance effects when PGR is used as a pharmaceutical composition.

Description

technical field [0001] The invention relates to a protein related to endometriosis and its application in endometriosis. The protein is PGR. The invention belongs to the field of biomedicine, specifically to the technical field of molecular biology. Background technique [0002] Endometriosis syndrome (EMS) is a common gynecological disease with the onset site located in the ovary and peritoneum. The cause of the disease is that the fallopian tubes plant active endometrial cells outside the endometrium (such as the pelvic cavity). Among women of reproductive age, the prevalence of EMS is about 10%-15%, and there are basically no patients before puberty and after menopause. The main pathological changes of endometriosis are periodic bleeding of ectopic endometrium and fibrosis of surrounding tissues, forming ectopic nodules. Patients are prone to symptoms such as dysmenorrhea, chronic pelvic pain, abnormal menstruation, and infertility. The etiology of endometriosis is co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/6883A61K48/00A61K38/17A61P15/00A61P15/08
CPCA61K38/1796A61K48/005A61P15/00A61P15/08C07K14/721C12Q1/6883C12Q2600/158
Inventor 李丽丽刘佳利黄娅
Owner 湖南省科域生物医药科技有限公司
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