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Biologic environmental-friendly diesel cleaning synergist composition

A technology of biological environmental protection and synergist, applied in the directions of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problem of no diesel cleaning and dispersing effect, and achieve good effects, broad market potential, and convenient use. Effect

Inactive Publication Date: 2020-08-11
上海聚茂塑胶制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no microbial agent for the detergency and dispersibility of diesel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of the bio-environmental diesel oil cleansing synergist composition, its steps are as follows:

[0037] (1) Preparation of mixed strains:

[0038] The Pseudomonas CCTCC No:M 2012281 and Rhodothermophilus-BNCC210143 strains were respectively activated and cultivated to a cell concentration of 10 8 cfu / g becomes the bacterial liquid, mixes the bacterial liquid of Pseudomonas CCTCC No:M 2012281 of 1 times the mass and the bacterial liquid of Rhodothermophilus marinum-BNCC210143 of 2 times the mass to obtain the mixed strain;

[0039] (2) Liquid wax induction culture:

[0040] Transfer to liquid wax induction medium, the inoculum size is 10%, shake culture for 24 hours;

[0041] The liquid wax induction medium:

[0042] 2% liquid wax, 1.5% yeast powder, 1% sucrose, 0.5% trehalose, 0.1% sodium dihydrogen phosphate, 0.1 potassium dihydrogen phosphate, pH 7.2;

[0043] (3) After liquid wax induction culture, centrifuge and evenly stir the obtained th...

Embodiment 2

[0051] The preparation method of the bio-environmental diesel oil cleansing synergist composition, its steps are as follows:

[0052] (1) Preparation of mixed strains:

[0053] The Pseudomonas CCTCC No:M 2012281 and Rhodothermophilus-BNCC210143 strains were respectively activated and cultivated to a cell concentration of 10 8 cfu / g becomes the bacterial liquid, mixes the bacterial liquid of Pseudomonas CCTCC No:M 2012281 of 1 times the mass and the bacterial liquid of Rhodothermophilus-BNCC210143 of 2.2 times the mass to obtain the mixed strain;

[0054] (2) Liquid wax induction culture:

[0055] Transfer to liquid wax induction medium, the inoculum size is 10%, shake culture for 36h;

[0056] The liquid wax induction medium:

[0057] 2% liquid wax, 1.5% yeast powder, 1% sucrose, 0.5% trehalose, 0.1% sodium dihydrogen phosphate, 0.1 potassium dihydrogen phosphate, pH 7.2;

[0058] (3) After liquid wax induction culture, centrifuge and evenly stir the obtained thallus;

[...

Embodiment 3

[0066] Control 1:

[0067] Preparation of bacteria-free diesel oil detergent synergist: 1 times the weight of Tween 80, 5 times the weight of oleic acid, 1 times the weight of trehalose, and 0.5 times the weight of magnesium chloride, mix evenly to obtain the bacteria mixed oily liquid.

[0068] Control 2: The preparation method is the same as in Example 1, only Pseudomonas CCTCC No: M 2012281 is used as the only fermentation strain.

[0069] Control 3: The preparation method is the same as that in Example 1, only using Rhodothermophilus marinum-BNCC210143 as the only fermentation strain.

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PUM

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Abstract

The invention relates to a biologic environmental-friendly diesel cleaning synergist composition. The biologic environmental-friendly diesel cleaning synergist composition contains pseudomonas CCTCC No:M 2012281 and rhodothermus marinus-BNCC210143. A using method of the diesel cleaning synergist comprises the following steps of mixing a biologic environmental-friendly diesel cleaning synergist with diesel and sodium chloride solution of 1% in the mass ratio being 1 to 100 to 100, performing an eddy stirring reaction at 50rpm for 24 hours, after the reaction, performing standing for 2 hours orabove, waiting for oil-and-water layering, and collecting an oil layer by a separating funnel. Oil products are modified cleaned diesel.

Description

technical field [0001] The invention relates to the application of microorganisms in the field of clean energy, in particular to a bio-environmental diesel cleaning synergist composition. Background technique [0002] Starting from January 1, 2019, the country will fully supply diesel oil that meets the National VI standard, and add diesel fuel detergent synergists that meet environmental protection requirements to vehicle diesel before sales. [0003] The increasingly stringent emission regulations require continuous innovation in diesel engine technology and continuous upgrading of diesel quality for vehicles. Adding diesel detergent to diesel can meet the diesel engine's requirements for diesel detergency. Diesel detergent can effectively remove nozzle carbon deposits and deposits, restore the original atomization performance and emission level of the engine, thereby indirectly reducing fuel consumption and reducing harmful gas emissions. [0004] Early diesel detergent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10L10/00C12R1/38C12R1/01
CPCC10L10/00C12N1/20
Inventor 李茂盛李德治赵鉴明
Owner 上海聚茂塑胶制品有限公司
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