Method for detecting urea and creatinine in forensic hemolytic blood sample

A technology in hemolyzed blood and samples, applied in the field of detection of urea and creatinine in forensic hemolyzed blood samples, can solve the problems such as the inability to obtain biochemical detection using blood samples, the inability to truly reflect the true concentrations of urea and creatinine, and the inability to perform detection, etc. To achieve the effect of reducing the detection error

Pending Publication Date: 2020-08-11
中国医科大学
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Problems solved by technology

[0003] For the pretreatment of hemolyzed blood samples, in the past, high-speed centrifugation and double dilution were used to process hemolyzed blood samples, but the liquid obtained after high-speed centrifuged hemolyzed blood samples still showed a serious red appearance, which could not be used for biochemical detection. Blood samples; after diluting the hemolyzed blood sample, although it can reduce the influence of blood color caused by hemolysis, it is found that urea and creatinine are not directly proportional to the dilution ratio, and if the concentration of the diluted sample is lower than The lower limit of the instrument detection method cannot be detected, which makes the blood sample after doubling dilution unable to truly reflect the true concentration of urea and creatinine in the original blood

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  • Method for detecting urea and creatinine in forensic hemolytic blood sample
  • Method for detecting urea and creatinine in forensic hemolytic blood sample
  • Method for detecting urea and creatinine in forensic hemolytic blood sample

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Embodiment Construction

[0034] like Figure 1-3 As shown, the ultrafiltration method uses a hollow fiber ultrafiltration module (MicroKros®, mPES, 50 000 D, 20 cm 2 , Spectrum Laboratories, USA) with disposable syringes and Amicon® Ultra-0.530KD vertical ultrafiltration components (Merck Millipore, USA) for ultrafiltration of hemolyzed samples, as detailed below.

[0035] 1. Assembly of the ultrafiltration device.

[0036] Assembly of the ultrafiltration device: the hollow fiber ultrafiltration module and 3 syringes as figure 1 Assembled as shown. Among them, No. 1 and No. 2 syringes are used as ultrafiltered blood samples, and No. 3 syringes are used as the first step of ultrafiltrate collection.

[0037] Second, the specific ultrafiltration process.

[0038] (1) Use the No. 1 syringe to draw 3 mL of the whole hemolyzed sample, repeatedly push the No. 1 and No. 2 syringes to perform the first ultrafiltration of the liquid in the ultrafiltration module, and use the No. 3 syringe to collect at lea...

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Abstract

The invention belongs to a blood sample detection method, and particularly relates to a method for detecting urea and creatinine in a forensic hemolytic blood sample. Color interference generated by hemoglobin in a hemolytic sample is avoided, and a conventional biochemical method can be more stable. The method comprises the following steps: step 1, performing ultrafiltration treatment on a totalhemolysis sample by using an ultrafiltration device to obtain ultrafiltrate; step 2, detecting urea and creatinine in the ultrafiltrate; 3, correcting the content values of urea and creatinine in theultrafiltrate; and step 4, obtaining the content values of urea and creatinine in the forensic hemolytic blood sample.

Description

technical field [0001] The invention belongs to a blood sample detection method, in particular to a method for detecting urea and creatinine in a forensic hemolyzed blood sample. Background technique [0002] The concentration of urea and creatinine in the blood after death has important auxiliary diagnostic significance in the inference of the cause of death in forensic medicine such as renal failure, fatal high and low temperature injury, and hyperosmotic dehydration, especially in the postmortem diagnosis of renal failure. plays a very important role. At present, the detection of urea and creatinine in forensic postmortem examination is mainly based on the detection of its concentration in body fluids by biochemical methods such as kits combined with microplate readers and manual detection of isolated serum samples. However, in the practice of forensic medicine, the blood of the corpse is often unable to separate the serum that meets the detection requirements due to hem...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N33/70G01N33/62
CPCG01N1/34G01N33/70G01N33/62
Inventor 曹志鹏贾宇晴王天琦田美慧朱宝利官大威
Owner 中国医科大学
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