Method for establishing rat cerebral hemorrhage model by combining ultrasound with microbubbles

Abstract

The invention relates to a method for establishing a rat cerebral hemorrhage model by combining ultrasound with microbubbles, and belongs to the technical field of biological model preparation. The method is implemented by the following steps of 1) taking one SPF-grade male SD rat with the body mass of 240-270 g; 2) making preparation work; 3) with the help of an auxiliary tool, injecting a microbubble contrast agent from the tail vein of the rat, wherein the injection amount is determined according to the body mass of the rat; 4) performing ultrasonic intervention; 5) carrying out CT plain scanning on the head of the rat; 6) irradiating the rat with an incandescent lamp for at least 2 hours, and keeping the anal temperature at 37+ / -0.5 DEG C; and 7) scoring the neurological function of the rat, and feeding and observing the rat for later use. According to the method, the contrast agent is ultrasonically targeted, and local blood vessels in an intracerebral target area are destroyed byvirtue of the instantaneous cavitation effect of the microbubbles, so that the rat has an intracerebral parenchymal hemorrhage phenomenon, and the research requirement of spontaneous cerebral hemorrhage is met; the method has the advantages of noninvasiveness, simplicity, convenience and easiness in popularization and is a proper reproducible biological modeling method; and meanwhile, auxiliary equipment is introduced, so that the operation is simpler, more convenient and more feasible.

Description

technical field [0001] The invention relates to a method for establishing a rat cerebral hemorrhage model in combination with ultrasound and microbubbles, and belongs to the technical field of biological model preparation. Background technique [0002] Intracerebral Hemorrhage (ICH) is a common cerebrovascular disease in neurology. It has the characteristics of high morbidity, disability, recurrence, and mortality. The incidence is increasing year by year, and the age of onset tends to be younger. The clinical treatment of cerebral hemorrhage is mostly based on dehydration to reduce intracranial pressure, surgical decompression and rehabilitation exercise, but most patients still have different degrees of neurological dysfunction, causing serious medical and economic burdens on families and society, and affecting the quality of life of patients and families . Therefore, only by establishing an animal model of cerebral hemorrhage with high reproducibility, good stability, an...

AI Technical Summary

Problems solved by technology

Collagenase-induced cerebral hemorrhage model is a kind of diffuse bleeding, the hematoma takes a long time to form, does not have an acute mass effect, and collagenase has cytotoxicity, and the inflammatory reaction around the hematoma is very heavy, which can directly destroy the blood brain This is different from the damage to brain tissue caused by simple spontaneous cerebral hemorrhage. Therefore, it cannot completely simulate the pathophysiological process of secondary brain injury after spontaneous cerebral hemorrhage in humans, and is not suitable for the treatment of brain tissue around hematoma. Research on tissue blood circulation disorders has limitations

The intracerebral hematoma formed by autologous blood injection into the cerebral hemorrhage model is not caused by the rupture of blood vessels in the brain. The volume of the hematoma is not directly related to the shape of the hematoma. If the injection is too fast, blood will flow back and overflow, and if it is too slow, the blood will coagulate in the needle. The pressure of the hematoma in the brain is also difficult to fix, and it is easy for the blood to swell and split the brain tissue to flow into the ventricle to form an intraventricular hematoma, so the formed hematoma has poor repeatability in shape and size

The disadvantage of the animal model of spontaneous cerebral hemorrhage is that it is prone to breakage and mutation, and there are limitations in genetics, and it is difficult to raise, the production cost is high, and there are few sources of acquisition.

[0005] Spontaneous cerebral hemorrhage occurs due to intracerebral blood vessel rupture and bleeding. None of the above methods can simulate the pathological process. At the same time, there are problems such as high technical requirements, invasiveness, and inability to standardize the modeling process.