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A cell-penetrating antimicrobial peptide targeting Streptococcus agalactiae and its preparation method and application

A technology of Streptococcus lactis and antimicrobial peptides, which is applied in the field of cell-penetrating antibacterial peptides targeting Streptococcus agalactiae and can solve the problems of difficult antimicrobial peptide linking and large hydrophobicity of short-chain hydrophobic peptides to achieve cytotoxicity The effect of small and large application prospects

Active Publication Date: 2022-02-08
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high hydrophobicity of short-chain hydrophobic peptides, it is difficult to directly link with antimicrobial peptides and play a role

Method used

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  • A cell-penetrating antimicrobial peptide targeting Streptococcus agalactiae and its preparation method and application
  • A cell-penetrating antimicrobial peptide targeting Streptococcus agalactiae and its preparation method and application
  • A cell-penetrating antimicrobial peptide targeting Streptococcus agalactiae and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0014] A preparation method of cell-penetrating antibacterial peptide L10 targeting Streptococcus agalactiae is as follows:

[0015] (1) Select the short-chain hydrophobic peptide of Streptococcus agalactiae as the targeting region, modify the 213-220 fragment of human extracellular superoxide dismutase rich in positive charges to form a cell-penetrating domain, and connect the two to obtain L10;

[0016] (2) Using a solid-phase chemical synthesis method to obtain a peptide resin through a peptide synthesizer, and cleaving the obtained peptide resin with TFA to obtain a polypeptide;

[0017] (3) After purification by reverse-phase high-performance liquid chromatography and identification by mass spectrometry, the preparation of the antimicrobial peptide L10 is completed, and its sequence is shown in SEQ ID No.1. The sequence and physicochemical parameters of antimicrobial peptide L10 are shown in Table 1.

[0018] Table 1 The sequence and physical and chemical parameters of ...

Embodiment 2

[0021] 1. Antibacterial activity

[0022] The peptides were prepared as a 2.56mM stock solution for later use. The minimum inhibitory concentrations of several antimicrobial peptides were determined by the broth microdilution method. Using 0.01% acetic acid (containing 0.2% BSA) as the diluent, a series of gradient antimicrobial peptide solutions were sequentially prepared using the double dilution method. Take 100 μL of the above solution and place it in a 96-well cell culture plate, then add an equal volume of the bacteria solution to be tested (~10 5 individual / mL) in each well. Positive controls (containing bacterial fluid but not antimicrobial peptides) and negative controls (neither bacterial fluid nor peptides) were set up. Incubate at a constant temperature of 37°C for 18 hours, and the minimum inhibitory concentration is the one where no turbidity is seen at the bottom of the well with the naked eye. The results are shown in Tables 2 and 3.

[0023] Table 2 Antib...

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Abstract

The invention provides a cell-penetrating antimicrobial peptide targeting Streptococcus agalactiae, a preparation method and application thereof. The sequence of the antimicrobial peptide L10 is shown in SEQ ID No.1. In the present invention, the short-chain hydrophobic peptide in the community communication system of Streptococcus agalactiae is used as the targeting region of the targeting antimicrobial peptide, and the human extracellular superoxide dismutase 213-220 fragment rich in positive charges is used as the basic fragment. Form a cell penetrating domain, connect the two to construct a new antimicrobial peptide L10 with targeting Streptococcus agalactiae and cell penetrating ability, which shows a good targeting effect, and has little effect on other bacteria , has highly effective antibacterial activity against Streptococcus agalactiae. Antimicrobial peptide L10 has low cytotoxicity and can kill intracellular Streptococcus agalactiae at the same time, which improves its development potential to replace antibiotics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a cell-penetrating antimicrobial peptide targeting Streptococcus agalactiae, a preparation method and application thereof. Background technique [0002] With the increase in the misuse of antibiotics, the problem of drug resistance has been paid more and more attention. Antimicrobial peptides, as the most potential alternatives to antibiotics, have also attracted the attention of researchers. It is generally believed that the mechanism of action of antimicrobial peptides is to use positively charged residues to generate electrostatic interactions with bacterial cell membranes, and then insert hydrophobic new amino acids into the membranes, destroying the integrity of the membranes, leaking the contents, and killing the bacteria. It is precisely because of this unique mechanism of action that it is difficult for bacteria to develop resistance to antimicrobial peptides. Ho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K1/04C07K1/06A61K38/10A61P31/04
CPCC07K7/08A61P31/04
Inventor 单安山李家维
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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