Microfluidic nucleic acid testing card box and kit

Abstract

The invention is applicable to the technical field of nucleic acid testing, and provides a microfluidic nucleic acid testing card box and kit. The card box comprises a card box support, a reagent operation part, a soft connecting part, a flow path part and an amplification chamber, wherein the reagent operation part is arranged at the upper part of the interior of the card box support and is provided with a sample cracking cavity, a plurality of liquid storage cavities and a pump operation cavity; the reagent operation part is provided with a first micro-flow pipeline and a second micro-flow pipeline; one end of the first micro-flow pipeline is connected with the pump operation cavity; a third micro-flow pipeline and a plurality of fourth micro-flow pipelines are arranged on the flow pathpart; the amplification cavity is arranged below the flow path part; and a valve assembly on the soft connecting part can control the connection or disconnection between the pump operation cavity andthe sample cracking cavity and between the pump operation cavity and the corresponding liquid storage cavity as well as the connection or disconnection between the pump operation cavity and the amplification cavity. The card box is powerful in detection function, high in detection flux, high in detection speed, high in automation degree and easy and convenient to use.

Description

Technical field [0001] The invention belongs to the technical field of nucleic acid detection, and particularly relates to a microfluidic nucleic acid detection cartridge and a kit. Background technique [0002] At this stage, molecular diagnostic technology based on nucleic acid detection is more and more widely used in real life, mainly including clinical detection and analysis, food safety detection, environmental monitoring and other application fields. Among them, in clinical analysis and testing, it can provide effective technical support for early auxiliary screening and diagnosis, prevention, testing and medication, and prognosis evaluation of diseases. The nucleic acid detection process based on molecular biology generally includes the collection of pathogen samples, nucleic acid extraction of the samples, PCR amplification, signal analysis and result interpretation in the later stage. Traditional nucleic acid testing first needs to build a professional district PCR lab...

AI Technical Summary

Problems solved by technology

Domestic nucleic acid detection products using the concept of microfluidics mainly include the HPV typing detection platform launched by Beijing Bohui Innovation Company, but there are disadvantages such as complex supporting instruments, high cost, and long detection time

Boao's disc microfluidic platform uses loop-mediated isothermal amplification technology for nucleic acid detection, but the detection platform does not have integrated sample pretreatment, and the pretreatment operation needs to be done manually in the PCR laboratory or rely on other laboratory equipment , unable to get rid of the professional PCR laboratory

[0005] So far, other research institutions or researchers have proposed a variety of nucleic acid detection platforms, but there are defects of one kind or another. Some have no pre-processing function for nucleic acid extraction, some have no supporting equipment, and some cartridges have low detection throughput. Some microfluidic cartridges have complex designs and high costs, and some instruments have defects such as low parallel processing capabilities

The developed system cannot well meet the actual application requirements

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