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Compositions for treatment of malignant tumors and precancerous conditions, methods of use thereof and methods for manufacturing medicaments

A composition and compound technology, applied in antineoplastic drugs, chemical instruments and methods, compounds of Group 5/15 elements of the periodic table, etc., can solve the problems of not providing methods or compositions for curing or preventing cancer, and achieve The effect of inhibiting proliferation

Pending Publication Date: 2020-08-14
梧桐树健康科学公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these prior arts do not provide methods or compositions for curing or preventing cancer

Method used

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  • Compositions for treatment of malignant tumors and precancerous conditions, methods of use thereof and methods for manufacturing medicaments
  • Compositions for treatment of malignant tumors and precancerous conditions, methods of use thereof and methods for manufacturing medicaments
  • Compositions for treatment of malignant tumors and precancerous conditions, methods of use thereof and methods for manufacturing medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Cell lines and reagents, colon cancer HT29 cell line, breast cancer MDA-MB-231, glioma U87 and skin cancer A2058 cells were obtained from the American Type Culture Collection (ATCC). These cancer cells were trypsinized and resuspended in cryopreservation medium containing 90% medium consisting of Dalbecco's Modified Eagle's Media (DMEM) and 10% fetal bovine serum (FBS).

[0097] Human cervical carcinoma HeLa was supplemented with 10% DMSO and stored in liquid nitrogen until use. Prior to the experiment, cells were recovered from liquid nitrogen and incubated in DMEM containing 10% FBS in an atmosphere of 5% CO 2 grow down. When cells reached approximately 80% confluency, cells were trypsinized and subdivided at a 1:4 ratio. To grow cells without exposure to test compounds, DMEM supplemented with 3.7 g / L sodium bicarbonate and pH adjusted to 7.3, supplemented with 10% FBS, and cells maintained at 37 °C in 5% CO 2 in the incubator. To study the pH-dependent and dose-d...

Embodiment 2

[0099] Effects of zinc pyrithione (Pyz) treatment at pH 7.4 and pH 6.4 on cancer cell viability.

[0100] Malignant tumors establish a characteristic acidic extracellular pH of 6.0-6.9, whereas normal tissues have a neutral pH of 7.3-7.4 (Parks et al., 2013). This demonstrates the exposure of zinc pyrithione to four different types of human cancer cells from cervical cancer (HeLa cells), brain tumor (U87 cells), colon cancer (HT29 cells) and breast cancer (MDA-MB-231 cells) lead to a significant decrease in cell viability. Treatment with zinc pyrithione significantly reduced cell viability when treated in acidic media at pH 7.4 compared to when treated at pH 7.4. The chemical structure of zinc pyrithione is as figure 1 shown.

[0101] Cancer cells were seeded onto 96-well culture dishes at 2,000 / well in an atmosphere of 5% CO 2 Incubate overnight at 37°C in the presence of DMEM supplemented with 10% FBS. To study the pH-dependent anticancer effects of pyrithione and other...

Embodiment 3

[0104] Effect of pyrithione sodium (Pyn) treatment at pH 7.4 and pH 6.4 on cancer cell viability.

[0105] Zinc pyrithione or other heavy metals chemically conjugated to pyrithione have been proposed to elicit anticancer activity. On the other hand, metal-free pyrithione has also been proposed to confer anti-inflammatory and anti-infective activity. Therefore, an important next question in the development of a new anticancer agent therapy is whether pyrithione causes an acid-enhanced anti-proliferative effect, or whether zinc causes an acid-enhanced anti-proliferative effect. To answer this question, the effect of pyrithione sodium on the proliferation of cancer cells was investigated.

[0106] This demonstrates the exposure of zinc pyrithione to four different types of human cancer cells from cervical cancer (HeLa cells), brain tumor (U87 cells), colon cancer (HT29 cells) and breast cancer (MDA-MB-231 cells) lead to a significant decrease in cell viability. Treatment with ...

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Abstract

The invention describes compositions and methods of use for pyrithione compounds and pharmacologically acceptable salts thereof. The invention provides topical administration methods for treating skincancers, treating cervical cancer, treating other cancers, preventing skin cancer development from precancerous skin conditions, preventing cervical cancer development from chronic human papillomavirus (HPV) infection, preventing development of other cancers from chronic HPV infection. Also disclosed are compositions comprising at least one pyrithione compound, ethylenediaminetetraacetic acid (EDTA), ascorbic acid, any salt and chemical derivatives thereof, pyridoxine and any derivative thereof and pharmacologically acceptable buffers. In these compositions, pH of the compositions is adjustedto between 4.5 and 6.4.

Description

technical field [0001] Rapidly growing cancer cells produce acid due to their metabolism, and the released acid makes the environment around the cancer cell acidic. This makes the cancer environment more acidic than normal tissue. The present invention discloses a novel composition and therapeutic / preventive method to inhibit the growth / emergence of cancer cells in an acidic environment. Background technique [0002] Malignant tumors have become the leading cause of death worldwide. According to statistics from the International Agency for Research on Cancer, there were more than 14 million new cancer cases in the world in 2012, and it is expected to increase to more than 20 million by 2030; increased to more than 13 million people. [0003] Chemotherapy, surgery, and radiation are the most commonly used therapeutic strategies against malignant tumors. Despite significant advances in the field of cancer research, there are still many issues to be addressed with current t...

Claims

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Application Information

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IPC IPC(8): A61K31/675A61K31/4415A61K31/4425A61P35/00C07D213/67C07D213/89C07F9/58
CPCA61K45/06A61K31/375A61K31/4415A61K31/555A61K31/675C07D213/89C07F9/58C07D213/67A61P35/00A61K9/0014A61K9/006A61K2300/00
Inventor 沼田雅行范泓逸李爱迪
Owner 梧桐树健康科学公司
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