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Treatment effect of monoclonal antibody on Parkinson's disease

A monoclonal antibody and Parkinson's disease technology, applied in the direction of antibodies, anti-animal/human immunoglobulin, nervous system diseases, etc., can solve the problems of no application, etc., to relieve pathological symptoms, alleviate changes in immune abnormalities, relieve Effects of Motor Behavior Disorders

Pending Publication Date: 2020-08-18
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antibodies against α-syn include antibodies against α-syn in different aggregation states (anti-monomer, anti-oligomer, anti-fibrosis antibody); α-syn is divided into three domains, N-terminal, NAC region and C-terminal, so there are also antibodies for recognizing different regions of α-syn (anti-N-terminal, anti-C-terminal, anti-NAC region antibodies), but none of them have been applied clinically at present

Method used

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  • Treatment effect of monoclonal antibody on Parkinson's disease
  • Treatment effect of monoclonal antibody on Parkinson's disease
  • Treatment effect of monoclonal antibody on Parkinson's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Comparison of p-α-syn content in cultured cell supernatant by indirect method ELISA

[0040] (1) Preparation of related reagents

[0041] A. Coating solution: CBS pH 9.6 500ml (Na2CO3 0.84g, NaHCO3 1.45g, H2O500ml) stored at 4°C.

[0042] B. Washing solution and dilution solution (mother solution): PBST pH 7.4 1000ml (10×) (NaH2PO4·2H2O 2.964g, Na2HPO4·12H2O 28.998g, NaCl2 9.000g, H2O 1000ml).

[0043] C. 20% Tween-20 100ml (Tween-20 20ml, H2O 80ml), autoclave, store at room temperature.

[0044] D. Washing solution and diluent (working solution): 50ml PBST pH 7.4, 1ml Tween-20 to a volume of 500ml.

[0045] E. Blocking solution: 5% BSA, 25ml (BSA1.25g, diluent 25ml, ready-to-use)

[0046] F. Substrate solution: OPD (o-phenylenediamine)-H2O2 preparation, phosphate-citric acid buffer 0.1M, take 4.86ml and 5.14ml respectively and mix

[0047]

[0048]

[0049] G. Chromogenic reagent (preparation for current use): take out 10ml F solution + 4mg OPD + 5μl H 2 ...

Embodiment 2

[0090] 1. CD4 in the plasma of transgenic mice + ,CD8 + T cell content detection

[0091] (1) Take blood from the inner canthus of the mouse, add 3 times the volume of erythrocyte lysate, centrifuge at 2,000g for 15 minutes, discard the supernatant, then add 2 times the volume of erythrocyte lysate, resuspend and centrifuge again at 2,000g for 15 minutes, discard the supernatant and save the precipitate .

[0092] (2) For Live dead staining, add 0.1 μL Zombie Green TM Dye to each tube, shake and mix well, incubate at room temperature in the dark for 15 minutes, wash with Wash Buffer, centrifuge at 1500g for 5 minutes, and discard the supernatant.

[0093] (3) Block Fc receptors: add 1 μg / 10 6 Cell Anti-Mouse CD16 / CD32 antibody blocked non-specific staining caused by fluorescent antibody Fc receptor. Incubate at 4°C with shaking for 30min.

[0094] (4) Add the corresponding surface-staining antibody to each tube, and incubate at 4°C for 30 minutes in the dark with shaking....

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Abstract

The invention relates to a treatment effect of a monoclonal antibody on Parkinson's disease, wherein the monoclonal antibody is an anti-serine 129 site phosphorylated alpha synuclein (p-alpha-synuclein, p-alpha-syn) monoclonal antibody (C140S). The invention particularly relates to an effect research of the antibody in treatment of abnormal activation of immune cells.

Description

technical field [0001] The invention relates to a therapeutic effect of a monoclonal antibody on Parkinson's disease, the monoclonal antibody is an anti-serine 129 phosphorylated alpha synuclein (p-α-synuclein, p-α-syn) monoclonal antibody ( C140S), the present invention particularly relates to the study of the role of this antibody in the treatment of abnormal activation of immune cells. Background technique [0002] Parkinson's Disease (PD) is a common neurodegenerative disease, mainly manifested as motor symptoms such as slow movement, muscle stiffness, resting tremor and postural instability, while other neuromodulation and neurotransmitter systems Also affected, causing various non-motor symptoms such as cognitive decline, depression, sleep disturbance, olfactory disturbance, urination, and gastrointestinal abnormalities; the pathogenesis of Parkinson's disease is not fully understood, and the main pathological features It is the progressive death of dopaminergic neuro...

Claims

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Application Information

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IPC IPC(8): A61K39/35A61P25/16
CPCA61K2039/505A61P25/16C07K16/18
Inventor 杨慧高歌
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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