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Preparation method of portable antibiotic biosensor

A biosensor and portable technology, applied in the fields of biology and chemistry, can solve the problems of low selectivity of immunoassay analysis technology, inapplicability of rapid detection and analysis, cumbersome sample pretreatment process, etc., to achieve good packaging effect, temperature and environmental adaptability The effect of strong ability and fast detection speed

Pending Publication Date: 2020-08-25
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, immunoassay has low selectivity of analytical technology, and false positive results may appear during sample detection; although microbiological assay is simple to operate and low in price, it has low detection sensitivity and takes a long time; chromatography has good accuracy and high sensitivity, However, the operation procedures are complicated, the price is expensive, and the sample pretreatment process is cumbersome, so it is not suitable for rapid detection and analysis

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  • Preparation method of portable antibiotic biosensor
  • Preparation method of portable antibiotic biosensor

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Embodiment 1

[0031] Embodiment 1: Portable biosensor is used for the detection of AMP

[0032] (1) Preparation of mesoporous silicon (MSN): to 2.0mg mL -1Add 875 μL of 2.0 M NaOH solution to the CTAB aqueous solution, and stir vigorously at 80° C. for 20 min. Subsequently, 1.25 mL of TEOS was added to the above solution, and stirred slowly at room temperature for 2 h. The obtained product was suction filtered, washed and dried. Take 164 mg of the precipitate and reflux in a mixture of 1.5 mL of hydrochloric acid (37%) and 75 mL of methanol for 10 h to remove the surfactant template, and the obtained MSN is suction-filtered, washed and dried at 60°C.

[0033] (2) Preparation of APTES-modified MSN: Take 25 mg of MSN prepared in step 1, ultrasonically disperse in 1.0 mL of absolute ethanol, then add 0.8 mL of APTES, stir slowly at room temperature for 6 h, centrifuge at 5000 rpm for 5 min and wash with absolute ethanol Wash 3 times, remove the supernatant, and obtain PMSN.

[0034] (3) Pr...

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Abstract

The invention belongs to the field of biology and chemistry, and relates to a preparation method of a portable antibiotic biosensor, which is used for conveniently and efficiently detecting antibioticAMP. The method is used for detecting oxidation-reduction reaction in an electrode tank, preparing a Glucose@MSN@ssDNA nano-storage, packaging an electronic medium glucose in an aperture, and sealingan aptamer chain (ssDNA) of a target AMP. The target AMP and the aptamer chain of the target AMP are specifically recognized, so that the aptamer chain is separated from the surface of the MSN, glucose in the hole is released and reacts with glucose oxidase modified on the detection electrode to generate current, the reading of the glucometer is changed, and AMP detection is realized. In the detection process of the sensor, a sample does not need to be separated, the assembly process is simple and convenient, the experiment cost is low, the accuracy is good, the selectivity is high, and the detection speed is high, so that the portable biosensor based on the DNA aptamer designed by the invention can be used for rapidly and sensitively detecting AMP.

Description

technical field [0001] The invention belongs to the fields of biology and chemistry, and relates to a preparation method of a portable antibiotic biosensor. Background technique [0002] Blood Glucose Meter (PGM) is an instrument that characterizes the health status of blood sugar by detecting the concentration of glucose in human blood. The signal is converted into an electrical signal that can be quantitatively measured, thereby realizing the determination of blood sugar concentration. Blood glucose meter detection is very important in the medical field and has many advantages: fast detection speed, accurate results, less use of test samples (only 1-5μL for one test), no need for pretreatment of samples, simple operation, small size and easy to carry , Experimental cost is low, so it is widely used in the medical field, used for real-time monitoring of diabetic patients. [0003] Aptamers generally consist of dozens of nucleotides, which can be RNA or single-stranded DNA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271G01N27/3277G01N27/3278
Inventor 李峰盖盼盼蒲黎
Owner QINGDAO AGRI UNIV