Primers for identifying molecular markers of sorghum tannin Tan1 allelic variant genes as well as identification method and an application of primers

Abstract

According to the invention, Tan1 has four allele types in total, namely Tan1, tan 1-a, tan 1-b and tan 1-c. The invention discloses a method for identifying a sorghum tannin allelic variant gene Tan1.The primers Tan1-BZF1 and Tan1-BZR1 are used for amplifying a sorghum Tan1 gene part open reading frame, the sizes Tan1 and tan1-a of PCR product fragments are 257 bp, and the sizes of tan1-b and tan1-c products are 267 bp and 247 bp respectively; tan1-BZF2 and Tan1-BZR2 are used for amplifying a sorghum Tan1 gene part, and a reading frame is opened; compared with Tan1, Tan1-a has the advantagesthat 580-bit G of a reading frame is deleted, a new EcoN1 restriction enzyme cutting site is generated due to deletion, a 344bp band and a 454bp band are generated after PCR fragments are subjected toEcoN1 restriction enzyme cutting, and identification of Tan1 and Tan1-a is completed. The invention develops an efficient molecular marker, and provides a more perfect and accurate detection means for cultivating special sorghum materials for food and feed with low tannin content.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a primer for identifying a molecular marker of a sorghum tannin Tan1 allelic variant gene, an identification method and an application. Background technique [0002] Tannins, commonly called condensed tannins (CT) or proanthocyanidins (PAs), widely exist in the plant kingdom, such as apples, blueberries, strawberries, grapes, cranberries, peaches and plums and other fruits and Tannins are found in the grains of sorghum, amaranth, faba beans, and barley, but not in the grains of major cereal crops such as rice, wheat, and corn. Tannins have multiple biological functions, such as antibacterial, anti-viral, anti-allergic, anti-oxidant, anti-inflammatory, anti-tumor, immune-promoting, ultraviolet protection, and preventing insects and birds from eating grains. Sorghum is one of the five major food crops in the world and has a wide range of uses, mainly for food, feed, brewing and bi...

AI Technical Summary

Problems solved by technology

Compared with Tan1, tan1-c loses its function due to the deletion of 10 bp bases in the coding region, resulting in frame shift and early termination of the reading frame

At present, there are no molecular markers that can quickly and effectively identify the four allelic variations of Tan1, tan1-a, tan1-b and tan1-c, which limits the organic combination of sorghum tannin molecular breeding and conventional breeding

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