Primers for identifying molecular markers of sorghum tannin Tan1 allelic variant genes as well as identification method and an application of primers
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of allelic variation and molecular markers, applied in the field of molecular biology, can solve the problems of loss of function, restriction of organic combination of sorghum tannin molecular breeding and conventional breeding, and achieve the effect of accurate detection means
Pending Publication Date: 2020-09-01
LIAONING ACAD OF AGRI SCI
View PDF1 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Compared with Tan1, tan1-c loses its function due to the deletion of 10 bp bases in the coding region, resulting in frame shift and early termination of the reading frame
At present, there are no molecular markers that can quickly and effectively identify the four allelic variations of Tan1, tan1-a, tan1-b and tan1-c, which limits the organic combination of sorghum tannin molecular breeding and conventional breeding
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0024] A primer for molecular markers to identify the allelic variant gene of sorghum tannin Tan1, the primers for the molecular markers are Tan1-BZF1 and Tan1-BZR1, and the primers for the restriction amplification polymorphic sequence method are Tan1-BZF2 and Tan1- BZR2, the sequence of the primer Tan1-BZF1 is SEQ1, the sequence of the primer Tan1-BZR1 is SEQ2, the sequence of the primer Tan1-BZF2 is SEQ3, and the sequence of the primer Tan1-BZR2 is SEQ4;
[0025] Wherein, SEQ1: 5'-CTCTGACCTCCGCTATATGG-3',
[0026] SEQ2: 5'-CTGCGTCGTACACTAGCACA-3';
[0027] SEQ3: 5'-TCTCCTTCGACCACCCCCTA-3',
[0028] SEQ4: 5'-AAGGCGATGGCCATCCAGTC-3'.
[0029] Specifically, the primers are designed using the following methods: figure 1 and figure 2 As shown, the full-length open reading frame of sorghum Tan1 gene was amplified with primers Tan1-F and Tan1-R, in which tan1-a had two mutations compared with Tan1, 580 G bases were deleted and 798 G was mutated to T , leading to changes in t...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
According to the invention, Tan1 has four allele types in total, namely Tan1, tan 1-a, tan 1-b and tan 1-c. The invention discloses a method for identifying a sorghum tannin allelic variant gene Tan1.The primers Tan1-BZF1 and Tan1-BZR1 are used for amplifying a sorghum Tan1 gene part open reading frame, the sizes Tan1 and tan1-a of PCR product fragments are 257 bp, and the sizes of tan1-b and tan1-c products are 267 bp and 247 bp respectively; tan1-BZF2 and Tan1-BZR2 are used for amplifying a sorghum Tan1 gene part, and a reading frame is opened; compared with Tan1, Tan1-a has the advantagesthat 580-bit G of a reading frame is deleted, a new EcoN1 restriction enzyme cutting site is generated due to deletion, a 344bp band and a 454bp band are generated after PCR fragments are subjected toEcoN1 restriction enzyme cutting, and identification of Tan1 and Tan1-a is completed. The invention develops an efficient molecular marker, and provides a more perfect and accurate detection means for cultivating special sorghum materials for food and feed with low tannin content.
Description
technical field [0001] The invention relates to the field of molecular biology, in particular to a primer for identifying a molecular marker of a sorghum tannin Tan1 allelic variant gene, an identification method and an application. Background technique [0002] Tannins, commonly called condensed tannins (CT) or proanthocyanidins (PAs), widely exist in the plant kingdom, such as apples, blueberries, strawberries, grapes, cranberries, peaches and plums and other fruits and Tannins are found in the grains of sorghum, amaranth, faba beans, and barley, but not in the grains of major cereal crops such as rice, wheat, and corn. Tannins have multiple biological functions, such as antibacterial, anti-viral, anti-allergic, anti-oxidant, anti-inflammatory, anti-tumor, immune-promoting, ultraviolet protection, and preventing insects and birds from eating grains. Sorghum is one of the five major food crops in the world and has a wide range of uses, mainly for food, feed, brewing and bi...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more