Horse embryo refrigerating fluid formula

Abstract

The invention discloses a horse embryo refrigerating fluid formula. The formula comprises serum, a sugar solution, a base solution, an inorganic salt solution and an anti-freezing solution, the freezing solution is prepared by mixing serum, the sugar solution, the base solution, the inorganic salt solution and the anti-freezing solution, wherein the serum ratio is set to be 5%-27%, the sugar solution accounts for 4%-8%, the basic solution accounts for 15%-30%, the vinylon fibers account for 5%-10%, and the anti-freezing solution accounts for 10%-25%. According to the invention, mutual cooperation, mutual support and synergistic effect among the serum, the sugar solution, the base solution, the inorganic salt solution and the anti-freezing solution are fully utilized; according to the present invention, the culture medium can achieve efficient permeation resistance and promote the integrity of the cell structure, further has a variety of energy matrixes, amino acids, vitamins, hormones,growth factors and cell factors, can be used for freezing sampled horse embryos as the embryo nutrition and energy substrate, and has high development rate after the frozen sampled embryos are frozen.

Description

Technical field [0001] The invention relates to the technical field of embryo engineering, in particular to the formula of horse embryo freezing liquid. Background technique [0002] An embryo is a larva in the first two months of pregnancy. The early development of blastocysts and embryos and embryonic development are a continuous process. During the somit period, all three germ layers have changed. The ectoderm is sunken into a groove on the midline of the back, called the nerve groove, and the two sides of the groove are called the nerve ridge. The nerve ridge gradually approaches and heals, causing the nerve to evolve into a neural tube running through the embryonic body. The neural tube and neural ridge will evolve into the nervous system in the future. The endoderm curls into a tube along with the embryonic body, called the archgut. The archgut is divided into foregut, midgut and hindgut according to the location. [0003] Embryo engineering refers to a variety of microm...

AI Technical Summary

Problems solved by technology

[0004] The effects and procedures of different cryoprotectants and their combinations in the freezing of embryos of different species are quite different. It is necessary to optimize the formula of cryoprotectant and the ratio of cryoprotectant and the corresponding freezing procedure in the application of specific species. At present, the glass freezing technology of horse embryos It is still not perfect, especially the freezing effect on in vitro embryos is poor. The overall functionality of the traditional freezing liquid formula is poor, the freezing effect on cells is poor, and the production cost is high. Therefore, here we propose the horse embryo freezing liquid formula