Unlock instant, AI-driven research and patent intelligence for your innovation.

Fluorescent glucose sensor based on glucose oxidase and pistol-like deoxyribozyme

A technology of glucose oxidase and glucose sensor, applied in the field of glucose sensor and fluorescent sensor, can solve problems such as pain and discomfort of patients

Inactive Publication Date: 2020-09-04
JILIN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection method is a blood test, which requires acupuncture to take blood, which is likely to cause pain and discomfort to the patient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent glucose sensor based on glucose oxidase and pistol-like deoxyribozyme
  • Fluorescent glucose sensor based on glucose oxidase and pistol-like deoxyribozyme
  • Fluorescent glucose sensor based on glucose oxidase and pistol-like deoxyribozyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The instrument used in the experiment of the present invention is a Tecan Infinite F200 multifunctional microplate reader.

[0024] The glucose oxidase used was purchased from Sigma, and the pistol-like deoxyribozyme and its DNA substrate were purchased from Sangon Bioengineering (Shanghai) Co., Ltd.

[0025] The fluorescent glucose sensor of the present invention will be further described below through specific embodiments.

[0026] Fluorescent glucose sensor for saliva sample detection:

[0027] The composition of the detection system includes 50 microliters of saliva sample, 0.01 unit / microliter of glucose oxidase, 50 nanomoles / liter of pistol DNase, 50 nanomoles / liter of pistol DNase substrate, 800 mmol / liter of chloride Sodium, 50 mmol / L MES buffer (pH 6.0) in a total volume of 100 µl.

[0028] After reacting in a water bath at 23°C for 15 minutes, the detection solution was transferred to a 96-well fluorescence detection plate using a micropipette, and the fluor...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fluorescent glucose sensor based on glucose oxidase and pistol-like deoxyribozyme, and belongs to the field of biochemical detection. Fluorescent quenching molecules are chemically modified at the 5'-end of pistol-like deoxyribozyme, and the fluorescent quenching molecules and fluorescent molecules are chemically modified at the 5'-end and the 3'-end of a DNA substrate ofthe pistol-like deoxyribozyme respectively. When the detection liquid contains glucose, glucose oxidase catalyzes glucose to generate hydrogen peroxide and gluconic acid, hydrogen peroxide and copperions act to promote breakage of a pistol-like deoxyribozyme catalytic substrate, and a fluorescence signal is generated at the same time. The lower limit of glucose detection of the sensor is 50 nanomole / L, curve fitting between the glucose concentration and a fluorescence signal conforms to an Extreme equation, and specificity is shown for glucose detection. By using the method, the content of glucose in saliva can be detected.

Description

technical field [0001] The invention belongs to the field of biochemical detection and relates to a glucose sensor, in particular to a fluorescent sensor for detecting glucose content in saliva based on glucose oxidase and pistol-like deoxyribozyme. Background technique [0002] Diabetes has become an important chronic disease threatening human health worldwide. In China, its incidence rate is constantly increasing, and the number of patients has accounted for the first place in the world. In order to treat or prevent diabetes, diabetic patients and susceptible people need to constantly monitor the glucose content in their bodies in daily life. The detection method is a blood test, which requires acupuncture to take blood, which can easily cause pain and discomfort to the patient. In order to solve this problem, diabetes detection is developing rapidly towards non-invasive detection, and the detection objects are gradually shifting from blood to body metabolites such as sa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6441
Inventor 姜大志许家翠于文倩刘程程郭艳杰
Owner JILIN UNIV