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Method for identifying pathogenic bacteria carrying toxic factors in water body based on metagenome technology

A metagenomic and pathogenic bacteria technology, applied in the field of water ecological security, can solve the problems of limited amplification target gene primer specificity, long culture cycle, difficult pathogenic bacteria, etc., and achieve high reliability results

Pending Publication Date: 2020-09-04
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional pure culture method has a long culture period and complicated culture conditions, making it difficult to apply this method to large-scale ecological surveys; molecular detection methods based on PCR technology are severely limited by the specificity of primers for amplifying the target gene, and it is difficult to It is difficult to comprehensively identify the distribution of pathogenic bacteria in water bodies

Method used

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  • Method for identifying pathogenic bacteria carrying toxic factors in water body based on metagenome technology
  • Method for identifying pathogenic bacteria carrying toxic factors in water body based on metagenome technology
  • Method for identifying pathogenic bacteria carrying toxic factors in water body based on metagenome technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Sample collection and metagenomic sequencing used in the present invention

[0049] 1.1 Sample collection

[0050] The receiving rivers of two sewage treatment plants in Beijing, Qinghe River and Tonghui River, were selected as the research objects. The basic information of the two sewage treatment plants is shown in Table 1. In December 2017, March, June, and September 2018, the planktonic microorganisms in the water and the biofilm growing on the stones were sampled throughout the year. Set up 7 sampling points for each river, one of which is located at the outlet of the sewage plant, three points are located upstream of the outlet of the sewage plant, and three points are located downstream of the sewage outlet, and the distance between two adjacent sampling points is 200±20 meters . Due to the freezing of the Qinghe River in December 2017, no river water samples were collected. A total of 105 samples were collected, of which 49 were river water samples a...

Embodiment 2

[0059] Embodiment 2 identifies pathogenic bacteria containing virulence factors

[0060] 2.1 Microbial community structure analysis

[0061] As described in Example 1, the diversity between the planktonic microbial community and the attached biofilm samples of Qinghe River and Tonghui River was analyzed. The weighted Unifrac distance results are shown in Table 2. The results show that the difference between the planktonic microbial community and the attached biofilm samples is greater than the difference between the two rivers. The follow-up analysis focused on comparing the differences between planktonic microorganisms and attached biofilms.

[0062] Table 2 Analysis results of diversity among samples

[0063]

[0064] 2.2 Genome Assembly

[0065] Using MEGAHIT (v1.1.3) software to splice and assemble the original metagenomic data, combined with the three binning methods of MetaBAT 2, MaxBin 2.0 and CONCOCT, and applying the MetaWRAP binning process, based on completene...

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Abstract

The invention belongs to the field of water ecological safety, and particularly relates to a method for identifying pathogenic bacteria carrying toxic factors in a water body based on a metagenome technology. According to the method, through a metagenome sequencing technology and a gene assembling method, the types and the number of the toxic factors in a water body are determined on the gene level, and pathogen host information of the toxic factors is identified, so that pathogenic bacteria carrying the toxic factors in the water body and potential health risks of the pathogenic bacteria aredetermined. The method has the characteristics of high flux, quickness, simplicity and convenience, and is suitable for large-scale sampling investigation and research.

Description

technical field [0001] The invention relates to a method for identifying pathogenic bacteria in water bodies, in particular to a method for identifying pathogenic bacteria carrying virulence factors in water bodies based on metagenomic technology, and belongs to the field of water ecological security. Background technique [0002] Modern human production activities have degraded the quality of river water environment and degraded ecological functions, while corresponding ecological health risks will gradually increase. The identification of pathogenic bacteria in the aquatic ecological environment is an important content of ecological health risk assessment. The methods usually used to identify and detect pathogenic bacteria include pure culture method and molecular detection method based on PCR technology. The traditional pure culture method has a long culture period and complicated culture conditions, making it difficult to apply this method to large-scale ecological surv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B30/10G16B30/20G16B40/00
CPCG16B30/10G16B30/20G16B40/00
Inventor 柏耀辉毛冠男梁金松王巧娟马百文胡承志曲久辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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