Acousto-optical device and method

Abstract

The invention relates to a device for reducing the chromatic spread angle of light (7) diffracted by an acousto-optical element (1), said device comprising an acousto-optical element (1), arranged ina beam path of an incident light beam (2), which produces the diffracted light from the incident light beam (2) that starts from a virtual interaction point (50) of the acousto-optical element (1), characterised in that the device comprises two focusing optics (10, 20), the first focusing optics (20) being arranged in the beam path upstream of the acousto-optical element (1) and the second focusing optics (10) being arranged in the diffracted light (7), and a focus (40) of the incident light beam (2) being located downstream of the first focusing optics (20) in the acousto-optical element (1),and the virtual interaction point (50) being in the front focal point of the second focusing optics (10).

Description

technical field [0001] The present invention relates to the field of optical microscopy, in particular multiphoton microscopy. [0002] The invention relates to a device for reducing the dispersion angle of light diffracted on an acousto-optic element, comprising an acousto-optic element and two focusing optics. The invention also relates to a method for reducing the dispersion angle of light diffracted on an acousto-optic element. Background technique [0003] In many areas of optical microscopy, regardless of the method used, a major requirement is the provision of excitation light at one or more defined wavelengths. Depending on the microscopic method and / or sample type, one or more excitation beams may be required, which usually must have defined spectral properties. [0004] For example, in the field of fluorescence microscopy it is important to use light with a wavelength which excites fluorescence. Especially when the sample contains fluorescent substances with dif...

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Problems solved by technology

[0019] For applications in (multiphoton) microscopy, too large a CSA is problematic for two reasons: firstly, the different propagation directions of the colored components of the femtosecond light pulse are responsible for making The beam profile is elliptical and thus does not illuminate the entrance pupil of the excitation objective optimally, which in particular leads to poorer resolution or reduced effectiveness (when the entrance pupil is over-illuminated)

But these schemes are either quite expensive or inflexible (e.g. when the laser wavelength is changed)

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