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A technology of Chrysanthemum chrysanthemum and craftsmanship, applied in the field of plant cultivation, can solve the problems of being unable to meet production and market demand, low reproduction coefficient, long breeding time, etc., and achieve the effects of excellent antioxidant effect, long breeding time and strong adaptability
Inactive Publication Date: 2020-09-08
舒城县健祥杭白菊种植专业合作社
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The cultivation technique and method of chrysanthemum blooming out of season in the above-mentioned patent have the following disadva
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Embodiment 1
[0043] A kind of chrysanthemum cultivation technique, such as Figure 1-3 shown, including the following steps:
[0044] S1: Cultivate seedlings, cut the young axillary buds of Chrysanthemum chrysanthemum with good growth and no pests and diseases as explants, inoculate them into induction medium, and induce callus formation under the conditions of 25±1°C and light intensity of 3000 lux;
[0045] S2: Select seedlings, induce differentiation into seedlings, perform histochemical staining and molecular detection on resistant plants, select transgenic plants containing the insertion sequence of the target gene, and plant them;
[0046] S3: Seedling cultivation;
[0047] S4: Strong seedlings, the clustered seedlings with 2 to 5 root systems were inserted into 1 / 2MS solid medium, and the strong seedlings were cultivated under the conditions of 25±1°C and light intensity of 3000 lux to obtain leaf stretching and leaf growth. The color is dark green, the root system is strong, and ...
Embodiment 2
[0077] A kind of chrysanthemum cultivation technique, such as Figure 1-3 shown, including the following steps:
[0078] S1: Cultivate seedlings, cut the young axillary buds of Chrysanthemum chrysanthemum with good growth and no pests and diseases as explants, inoculate them into induction medium, and induce callus formation under the conditions of 25±1°C and light intensity of 3000 lux;
[0079] S2: Select seedlings, induce differentiation into seedlings, perform histochemical staining and molecular detection on resistant plants, select transgenic plants containing the insertion sequence of the target gene, and plant them;
[0080] S3: Seedling cultivation;
[0081] S4: Strong seedlings, the clustered seedlings with 2 to 5 root systems were inserted into 1 / 2MS solid medium, and the strong seedlings were cultivated under the conditions of 25±1°C and light intensity of 3000 lux to obtain leaf stretching and leaf growth. The color is dark green, the root system is strong, and ...
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Abstract
The invention discloses a chrysanthemum morifolium cultivation technology, and relates to the technical field of plant cultivation. In order to solve the problem of long breeding cycle, the chrysanthemum morifolium cultivation technology specifically comprises the following steps of cultivating seedlings, cutting out tender chrysanthemum morifolium axillary buds which are good in growth vigor andfree from diseases and insect pests as explants, inoculating induced culture media with the explants, and performing induction under the condition of 25+/-1 DEG C and light being 3000 Lux in light intensity to obtain callus tissue; and performing seedling selection, and performing induction and disintegration to obtain adult seedlings. According to the chrysanthemum morifolium cultivation technology disclosed by the invention, a process of bud proliferation and rooting culture is completed in one time, and the shortcomings that breeding cycle is long, breeding coefficients are low, and chrysanthemum morifolium seedlings are liable in virus infection and propagation during conventional cuttage and offshoot of chrysanthemum morifolium are overcome; potato natural extracts are used as the culture media, so that the disintegration and proliferation rate of chrysanthemum morifolium buds is increased through active factors in the extracts; chrysanthemum morifolium is planted by a root dividing method, and the root dividing method is suitable for large-area culture; and in a planting process, parameters in each link are set according to habits and characteristics of the chrysanthemum morifolium, so that efficient organism extraction can be performed, and time and cost can be saved.
Description
technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a cultivation technique of Chrysanthemum chrysanthemum. Background technique [0002] Hangbai chrysanthemum, also known as chamomile and white chrysanthemum, has strong adaptability, is more cold-resistant, and is not resistant to high temperature. The suitable temperature for growth is 15°C to 25°C. It grows best in loose, fertile, moist loam or sandy loam. , Chrysanthemum chrysanthemum is the dry flower head of Chrysanthemum chrysanthemum, which has a long history of cultivation. According to relevant historical records, it has been at least 360 years old. It is one of the authentic medicinal materials of "Eight Flavors of Zhejiang Province". 7-β-D-glucoside, apigenin-7-β-D-glucoside, acacetin-7-β-D-glucoside and other flavonoid active ingredients have excellent anti-oxidation effects The effect of wind, calming the liver and improving eyesight, early research findin...
Claims
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