Chrysanthemum morifolium cultivation technology
A technology of Chrysanthemum chrysanthemum and craftsmanship, applied in the field of plant cultivation, can solve the problems of being unable to meet production and market demand, low reproduction coefficient, long breeding time, etc., and achieve the effects of excellent antioxidant effect, long breeding time and strong adaptability
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Embodiment 1
[0043] A kind of chrysanthemum cultivation technique, such as Figure 1-3 shown, including the following steps:
[0044] S1: Cultivate seedlings, cut the young axillary buds of Chrysanthemum chrysanthemum with good growth and no pests and diseases as explants, inoculate them into induction medium, and induce callus formation under the conditions of 25±1°C and light intensity of 3000 lux;
[0045] S2: Select seedlings, induce differentiation into seedlings, perform histochemical staining and molecular detection on resistant plants, select transgenic plants containing the insertion sequence of the target gene, and plant them;
[0046] S3: Seedling cultivation;
[0047] S4: Strong seedlings, the clustered seedlings with 2 to 5 root systems were inserted into 1 / 2MS solid medium, and the strong seedlings were cultivated under the conditions of 25±1°C and light intensity of 3000 lux to obtain leaf stretching and leaf growth. The color is dark green, the root system is strong, and ...
Embodiment 2
[0077] A kind of chrysanthemum cultivation technique, such as Figure 1-3 shown, including the following steps:
[0078] S1: Cultivate seedlings, cut the young axillary buds of Chrysanthemum chrysanthemum with good growth and no pests and diseases as explants, inoculate them into induction medium, and induce callus formation under the conditions of 25±1°C and light intensity of 3000 lux;
[0079] S2: Select seedlings, induce differentiation into seedlings, perform histochemical staining and molecular detection on resistant plants, select transgenic plants containing the insertion sequence of the target gene, and plant them;
[0080] S3: Seedling cultivation;
[0081] S4: Strong seedlings, the clustered seedlings with 2 to 5 root systems were inserted into 1 / 2MS solid medium, and the strong seedlings were cultivated under the conditions of 25±1°C and light intensity of 3000 lux to obtain leaf stretching and leaf growth. The color is dark green, the root system is strong, and ...
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